FastQCFastQC Report
Sat 22 Oct 2022
EGAF00006810885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006810885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3930581
Sequences flagged as poor quality0
Sequence length75
%GC67

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCG496401.2629176195580247No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATG313540.7976937760600787No Hit
GTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCG290790.739814292085572No Hit
GTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTC271710.6912718501412386No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTC254370.6471562346635268No Hit
CCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGA197850.5033606990925769No Hit
GGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCC193110.49130141320074566No Hit
GGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTC179330.4562429829076159No Hit
GGGAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCC174430.4437766325131068No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160170.40749700871194355No Hit
CTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCG156080.39709142236224115No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAG152990.38922998915427515No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGA151280.3848794872819057No Hit
GTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGG147380.37495729002913314No Hit
CCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGAC144130.3666887923184893No Hit
GCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCG143390.3648061189936042No Hit
CCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCGCGGTGGAAATGC139400.3546549479580754No Hit
CGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTG138420.3521616778791736No Hit
GGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGC121760.3097760865378426No Hit
GCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGG117290.2984037219942802No Hit
GGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGG116470.29631751641805626No Hit
GGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCC116200.2956305950697874No Hit
GTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGG112370.2858864885369364No Hit
GGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACC111720.28423278899480764No Hit
CCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGGG111100.28265541404693095No Hit
GGGGAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGC104830.2667035738482428No Hit
GGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCC99950.2542881065165684No Hit
GTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTC99320.25268529003727436No Hit
GACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC95290.2424323528760761No Hit
GGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGC91750.23342605075432868No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGG88540.22525931916935438No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGG85950.2186699625322567No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGTCCTTCCTC83660.21284385183767995No Hit
GGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGG81570.20752657177145056No Hit
GCCCGCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCA80980.206025521417826No Hit
GGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCC79870.20320151143049847No Hit
GGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCG79370.20192943485963016No Hit
GGAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCC79140.20134427963703078No Hit
GGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTC78360.19935984018647623No Hit
GGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATC77450.19704466082749597No Hit
GTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCC77170.19633229794780976No Hit
GGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGG75860.19299945733213486No Hit
GGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCC75520.19213444526394444No Hit
GGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGT74850.19042986265898096No Hit
GACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGG71710.18244122179392816No Hit
GGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCAC71700.18241578026251082No Hit
CTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGA70610.17964265333801796No Hit
GGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGA70230.17867587514415806No Hit
CGGCGGGCGGCGGGCGGGGAAGAGGGCACAGACGGGCGAGGGCCGGGGACCGCGAGGGCAAGGGCACCCGGGAGC70200.17859955054990598No Hit
GGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCC69110.17582642362541312No Hit
GGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGG68570.17445258092887542No Hit
GGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCG66690.1696695730224107No Hit
CCCGACCCTTCTCCCCCCGCCGCCGCCCCCACGCGGCGCTCCCCCGGGGAGGGGGGAGGACGGGGAGCGGGGGAG65660.16704909528642203No Hit
GGCGGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGAC64890.1650900973672849No Hit
GGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACG63690.16203711359720102No Hit
GGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGC63480.16150284143743635No Hit
GCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCC63170.16071415396349803No Hit
CGGGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGC62950.16015444027231598No Hit
GACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCT60880.1548880432689213No Hit
CGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTC59020.15015591842529133No Hit
GGGAGGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAG58480.14878207572875357No Hit
GGGGCGGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCG58220.14812059591190208No Hit
GGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGC57780.14700116852953798No Hit
GGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATT56880.1447114307019751No Hit
GAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCC56780.14445701538780145No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGTCCTTCCTCCTCGCT55770.14188742071464752No Hit
CGGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACAC55200.14043725342385768No Hit
GTGGGGGGGAGGGCGCGCGACCCCGGTCGGCGCGCCCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCG54600.13891076153881576No Hit
CGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGC54300.1381475155962948No Hit
GGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCG53420.13590866083156664No Hit
GGCTGAGTGAAGCATTGGACTGTAAATCTAAAGACAGGGGTTAGGCCTCTTTTTACCAGCTCCGAGGTGATTTTC52280.13300832624998696No Hit
GACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCC51040.12985357635423364No Hit
GGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGC49960.12710589096115815No Hit
GGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCG49860.12685147564698449No Hit
GGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCG49030.12473982853934316No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA48960.1245617378194216TruSeq Adapter, Index 7 (97% over 36bp)
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGTCCTTC47990.12209390927193715No Hit
GCGGCGGGGGAAGGGAGGGCGGGTGGAGGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACA47120.11988049603862636No Hit
GGGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCG47100.11982961297579162No Hit
CACCGTTCGGCCTCGGGCACCTGAGGGACAACCCGGAGCGCTCCAGGAGCACCGCAAGGGCCCAGGCGGAGCCGA46190.11751443361681137No Hit
GGGGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTC46160.11743810902255926No Hit
GGGAGGAGGAGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGA45520.11580985101184788No Hit
GACGGGTCGGGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGC45490.11573352641759578No Hit
GGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGC45390.11547911110342211No Hit
GGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGA44430.11303672408735502No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCGTCCTTCCTCCTCGC44430.11303672408735502No Hit
GGTGGGTAGCCGACGTCGCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGC44340.11280775030459873No Hit
GCCGCCGACCCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCC44080.11214627048774722No Hit
GACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCA43760.11133214148239153No Hit
GCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGC42500.10812650852380348No Hit
GTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCACCGTGGGACGCTTTCCCAGGGCCAGGCG42180.10731237951844777No Hit
GGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCCGGACCCGCCGCCGGGTTGAATCCTCCGGG41930.10667634123301364No Hit
GTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCG41810.10637104285600525No Hit
GGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCC41710.10611662754183161No Hit
GGCGCGGGGAGGAGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGG41680.1060403029475795No Hit
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCCGGGCCCGACGGCGC41660.10598941988474476No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA41620.10588765375907531No Hit
GCCACCACCACCGTCGCTCGTGATTCTCGTCCATCCTCCGACCCGGTCCCGCTCCGGGAGACCGGCGCGCCCCCA41350.10520073241080644No Hit
CGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGC41100.10456469412537231No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGG41020.10436116187403338No Hit
GACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACC40260.10242760548631359No Hit
CGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGAACCTCCCCCGGGCCCGACG40220.10232583936064414No Hit
CCGGCCCCCCCACGAGGAGACGCCGGCGCGCCCCCGCGGGGGAGACCCCCCTCGCGGGGGATTCCCCGCGGGGGT40060.10191877485796628No Hit
GGACGCGGGGCCGGGGGGCGGAGACGGGGGAGGAGGAGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCC39710.10102832125835849No Hit
GGAGGAGGAGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCC39550.10062125675568065No Hit
GGACGGACGGACGGACGGACGGACGGGGCCCCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGC39330.1000615430644986No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)