FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10390370
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATTCC349784833.66432571698601No Hit
TTGCCGGA287902327.708570532136967No Hit
AGTTGCAG174834916.826628888095417No Hit
CAAGAATT163535815.739170019931917No Hit
AGTTGCAT770090.741157437126878No Hit
CAATAATT324130.3119523173861951No Hit
GCCAATCC311970.30024917303233667No Hit
TGTTGCAG280560.2700192582169836No Hit
TTTCCGGA257750.2480662382571554No Hit
GCCATACC237900.2289620100150428No Hit
GCCATTCA217660.2094824342155284No Hit
GTGCCGGA209690.20181187002965245No Hit
CAAGAATA205190.19748093667501734No Hit
CAAGAAAT188070.18100414133471668No Hit
TAGCCGGA137410.13224745605786897No Hit
CAACAATT122420.11782063583876223No Hit
TTGCCGGT117190.11278712885104188No Hit
TTGCCGAA111830.10762850601085429No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)