FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9861837
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCCTC250440125.39487318640533ABI Solid3 Adapter A (100% over 8bp)
CCGGGTAT235954423.926008916999947No Hit
ATCATGCA228925423.21326138324939No Hit
TAACAAGG224119722.72595866267106No Hit
GGGTCCTC222710.22583013692073797No Hit
CCGGGTAA193170.19587628552368083No Hit
CCGGGGAT177090.17957100690266936No Hit
GAACAAGG170940.17333484623605114No Hit
TAACAATG165840.16816339592714827No Hit
GGTTCCTA159500.1617345733862768No Hit
TTCATGCA154140.15629948051260634No Hit
GGTTCCAC139650.14160647757613515No Hit
AACATGCA139380.14133269491272266No Hit
ATCAAGCA138570.14051134692248515No Hit
TAACAACG119280.1209510966364583No Hit
GGTACCTC114520.11612440968148227ABI Dynabead EcoP Oligo (100% over 8bp)
TAAAAAGG107590.10909732132056127No Hit
TAACAAGC100230.10163420871790925No Hit
TAACAAGA99070.10045795727509996No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)