FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13348248
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGAAC407912130.559223952087194No Hit
ACACTGTT292436721.90824593609588No Hit
CAGGATGG290913921.794163548654474No Hit
TTTACCCA276060720.68141826552818No Hit
GTTACCCA365310.27367636561742037No Hit
ACACAGTT316730.23728207626948494No Hit
ACACTGTA298650.22373722753727682No Hit
GGCTGTAC246840.18492314497003653No Hit
TCACTGTT246680.18480327905205238No Hit
GGCAGAAC226230.1694829164097041No Hit
GGCTGAAA221580.16599931316828995No Hit
CAGGAAGG214510.1607027379173656No Hit
TATACCCA191290.1433071965699169No Hit
ACCCTGTT190540.14274532507936621No Hit
TTTACCAA184460.13819042019596878No Hit
TTAACCCA171450.12844382273988317No Hit
TTTTCCCA142040.1064109686904229No Hit
ACACTGAT139530.10453057210204665TruSeq Adapter, Index 25 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)