FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7751110
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCAATT256081033.03797778640737No Hit
GATACGCC253058732.6480594392287No Hit
CTAGGTGA214335627.652245936388464RNA PCR Primer, Index 38 (100% over 8bp)
AGCCAAAT457480.5902122405694152No Hit
TTAGGTGA352280.45448974404956194No Hit
GAAACGCC313830.40488394565423536No Hit
GTTACGCC299990.387028438507517No Hit
AGCCAATA288170.37177900971602773No Hit
TGCCAATT268760.3467374350254351No Hit
TCGTTCAG238600.3078268789889448No Hit
CTAGGAGA192270.2480547947326254No Hit
GTAGGTGA191190.24666144590903752No Hit
ATCCAATT187540.24195244294043047No Hit
GATACGCA173230.2234905710278915No Hit
CAAGGTGA156510.2019194670182722Illumina PCR Primer Index 12 (100% over 8bp)
GATACCCC131930.17020787990365252No Hit
CTATGTGA110380.14240541032187648No Hit
ACCCAATT79650.10275947573960376No Hit
GATTCGCC79250.1022434206197564No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)