FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11300349
Sequences flagged as poor quality0
Sequence length8
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCCTCAT391705134.66309757335813No Hit
CCAGGGCA358356431.711976329226648No Hit
AATAATGG237930821.055172720771722No Hit
GTGTCATC8683097.683913125161002No Hit
TGCCTCAA363820.32195465821453834No Hit
GGCCTCAT336450.2977341673252747No Hit
TATAATGG247520.2190374828246455No Hit
TGCCACAT218670.19350729787195067No Hit
AAAAATGG207810.18389697521731407No Hit
AATAAAGG199710.17672905500529232No Hit
AATAATGA164280.14537604104085636No Hit
GCAGGGCA156820.13877447501842644No Hit
TGGCTCAT135750.12012903318295745No Hit
CCATGGCA132010.11681940088753011RNA PCR Primer, Index 34 (100% over 8bp)
TGCATCAT131030.1159521710347176No Hit
CCACGGCA128540.11374869926583683No Hit
CCAGAGCA126870.11227086880237062No Hit
TGCCTAAT123060.10889929151745667No Hit
AATAATGT118820.10514719501141072No Hit
AATAATTG114390.10122696210532965No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)