FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115949306
Sequences flagged as poor quality0
Sequence length91
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA2252750.19428749319120547Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA1960280.1690635388537815Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA1469070.1266993353112437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1467960.12660360381975896No Hit
CTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATATTTCACTTTA1335910.11521500611655233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)