FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68015361
Sequences flagged as poor quality0
Sequence length91
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGAGGGGGGGGTGGGGGGGGGGGGGGGGAGGGAGGGG34755225.109907451641696No Hit
CATCAACCAACCACACCTAGCATTCCTGCACATCTGTACCCACGCCTTCT34707555.102898740771221No Hit
GNGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA34612575.088934248250186No Hit
GCCGAGGCTATCAAGGCCCTCGTAAAGCCCAAGGANGTTAAGCCCAAGAT34561605.081440352863818No Hit
ANNCAGTGGTATCAACGCAGNGTACATGGGTACCATTGTTGAAAATCAAC34507855.0735377262792145Clontech SMARTer II A Oligonucleotide (95% over 22bp)
CAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCA34411585.05938357072015No Hit
CNTGTATGAGTGGAGACGGGCCTGGGTCTTGGGGACTGGAGGGCAGGGGT34363835.0523630978008045No Hit
ATTCTCCGATCCGTCCCTAACAAACTAGGAGGCGTCCTTGCCCTATTACT34350955.050469407932717No Hit
NNGCCTACCTCTAGTCCCAGCTACTCTGGAGGCTAAGGTGGGAGAATGGC34184405.025982292441262No Hit
GAAAAGACCAAATCTACATCTGATTGGTGTACCTGAAAGTGATGGGGAGA34132985.01842223553No Hit
GNNCAACATTTTTTNTAGCCNCAGGCTTCCACGGACTTCACGTCATTATT33975974.995337744366306No Hit
ANAATGTCAAGGCAAAGATCCAAGATAAGGAAGGCATCCCTCCTGATCAG33862254.978617991897448No Hit
CTCCCACACAAGTTCATAAACTCTTCAAAAAAGGATCTTTTTTTATTCAT33828694.9736838123964375No Hit
NTTTGAATCATATCCTCTAACTACTCTGTAAGAAACGTTTTTACATCAAT33746424.9615880153896414No Hit
CNNCGGCGCAGTCATTCTCATAATCGCCCACGGGCTTACATCCTCATTAC33682024.952119566049205No Hit
GNNAAAATTAGTTGTTTTCATGTATGTCAGCAAAACCCAGATTTCAGTCA33517124.927875042815696No Hit
ANNTCCTAGAGAGGAAGTTCCCTGAGTATGTGAGTAGGGCAGCCCTTCCT33516544.927789767961388No Hit
GNGCTGTAGGCACCTCTCGCCCCTTCTCTCATCAGCTTTCTCAGGCTATC33320494.89896539694908No Hit
ANGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC33151744.874154825113697Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CNGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCATGCACCTA32963844.8465287128300325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)