FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47287795
Sequences flagged as poor quality0
Sequence length91
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTACTATGCTATGTTGGCTAAAACTGGTGTCCATCACTACAGTGGCA34147967.221305201479579No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAACACGGGAAACCTCACCC34145817.220850538706658Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGTTCCTTCCAGTGTCATTTCTCATCCACATACCCTGACCTGGCCCCCTC34127067.216885456384676No Hit
CGTCCTAGAATTAATTCCCCTAAAAATCTTTGAAATAGGGCCCGTATTTA34094307.20995766455171No Hit
TAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCCCCACATTAGGC34089837.209012388926149No Hit
GNNGCTACAAAGGAGAGTAATAATCCTCAAGGTTGTGGAAATCATGTTAA34089707.208984897688716No Hit
CNNCGGAAATGCTCCGCTAGCAATCGCATCATCGGTGCCAAGGACCACGC33973087.184323143001276No Hit
ANCAGAGAGTTTGAATTTAGACATATATTTAGAAAGAGGAAAAGATGAAC33827497.153535071787551No Hit
NNNAGATCTTCGACACAGAAANANANAAATATGAGATTACGGAGCNGCGC33666057.119395184317645No Hit
CNNAAGAAGCTGATTGCAGCCCAAACTGGTACCCTTTGGAACAAGATTGT33649187.115827667583146No Hit
CNNGTCTGGGCAACAGAGCGAGACTCTGTCTCACACACAAAACAAAACAA33566757.098396108340429No Hit
ANATACAAATTTTTTGAGGTTATCCTCATTGATCCATTCCATAAAGCTAT33350367.052635886278901No Hit
TACACCACTCCCAAGAAGAATAAGCACAAGAGAAAGAAGGTTAAGCTGGC33141137.0083897969867275No Hit
NNNTTAAACTTCCTTCGTAGTGATCTCATTGAAGTTGCAACAAGGTTACA33009256.980500993966836No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)