FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121160978
Sequences flagged as poor quality0
Sequence length91
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAA34465272.8445849950138236No Hit
NGGTGTCCGCATGTTACGAGAGCGGGCTGCGTTTGTGTGGTGAATGGGGA34435062.8420916179795115No Hit
TTTTTTTAAAAAGTATTATGTTAATTGACAGAAGTCAGAAATAAGATATA34335572.833880228335562No Hit
CCAGCAGTTTGGGAGGCCGGGGTGGGTGGATCGCTTGAGCCCAGGAGTTT34316112.8322741006597023No Hit
NTTAGTGNTNGGCCTTCTCTTNANCNTNANTNCNANGNGGTTAGTNCNTT34280822.8293614467192563No Hit
CCCCAAGATCGGCCCCCTGGGTCTGTCTCCAAAAAAAGTTGGTGATGACA34276712.8290222285924433No Hit
NTAAGATATCTACTACAATGAAAGAGCCAAACTCAAAACCAGATTTTTTG34130102.8169217980396297No Hit
GATCCAGCCCGACCTGCGAGAGCTGATGGAGACCATGCACCGCATGAGCC34022362.808029496097333No Hit
NCCGCAGGCCGCCCTACCCACCTTCAGGCAGCCTATGGGACGCAGGGCCC34011852.80716205509665No Hit
NNNCAGTGGTATCAACGCAGANTNCNTGGGGNCCAGGACCCGCAGNAGAG33989802.8053421622265216No Hit
ANNCAGTGGTATCAACGCAGAGTACATGGGATGACCATCTCCCAGAACAG33980802.8045993488101426Clontech SMARTer II A Oligonucleotide (100% over 22bp)
NTGGTATCAACGCAGAGTACATGGGGCACTCTTTCTGACTACAACATCCA33953842.8023742099539675No Hit
NCAGGCCTAAGCAATCCTCCCNCNTNGNAGCNGNGACTACAGCTCNCNGC33936732.8009620391146064No Hit
AGCTGCCCTCCGGCTCCAAGAAGGTTATCTCCTCAGCCAACAGAGCTGTG33905002.7983432091477507No Hit
AGAATGGGTCTCTTGTTGAAATCCGAAATTTCTTGGGTGAAAAATACATC33890852.797175341387555No Hit
NTGGTATCAACGCAGAGTACATGGGCCTGTCTGAGTGCCGTGGATGCCCA33860502.794670409477877No Hit
TCCCCACTATGCACATTTTATTTCTCCAACATACTCGGATTCTACCCTAG33795872.7893361838000352No Hit
NTGGGTCACCAGCAGCTGTACTGGAGCCACCCGCGAAAATTCGGCCAGGG33792832.789085277934947No Hit
NTACATCCAGCACTGTAGTTAAGTACCCAAAATTGAATAGAAATAATGGC33781672.7881641892986373No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGGCACACCTACACCCCTT33748132.785395971300265Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTC33734862.784300734185226No Hit
NCAAAGAACAACACAGTCACTCCTGGCGGCAAACCAAACAAAACCAGAGT33727532.7836957539249974No Hit
NNCCCTAACAAACTAGGAGGCNTCCTTGCCCTATTACTATCCATCCTCAT33669982.778945874801374No Hit
GGTTGACAAGGTCACAGGCAGGTTTAATGGCCAGTTTAAAACTTATGCTA33660832.7781906811613886No Hit
GNAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCT33646372.7769972276057397No Hit
AGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAGGAGGAGACC33581352.7716308133465217No Hit
CCTGGACTGACTGATACTACAGTGCCTCGCCGCCTGGGCCCCAAAAGAGC33562572.7700808093510108No Hit
GTGGACAAGAGGATCCTGGATGCTGCAGGGGCCAATCTCAAAGTCATCAG33533462.7676782206231447No Hit
TNATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAGCCTCCTTAT33489792.7640739248572257No Hit
ANNAGTGGTATCAACGCAGAGTACATGGGTACTACACAATCAAAGACGCC33398942.7565756360930003Clontech SMARTer II A Oligonucleotide (100% over 21bp)
ATATTATGTAAAGACCACCCCTCCTCATTCCAGAACGAACAGCCTGACAC33120842.733622701526889No Hit
TATCAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCAATG33091362.731189574914128No Hit
TNTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTT33043332.727225427315385No Hit
ANCATAGAATTCTCACTGTGATATATAAACTCAGACCCAAACATTAATCA32207292.6582230130232194No Hit
GGAGCACGTGGCTGATGAGAACCTCAAGGCCTTCCTCAGGATGGAGATCC32184782.6563651541340314No Hit
ATTCGGGGAAAGAATTGAACCTTCACCATATAAGTTTACGTTTAATAAGA31046632.5624281441505037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)