FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006816879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006816879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37177053
Sequences flagged as poor quality0
Sequence length91
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GNAACCCCATCTCTACTAAAAATACAAAAATTAACTGGGCATGGTGACAC34442649.26448903843992No Hit
ACAGAGCTGGGAAAATTGTTGTGAACCTCACAGGCAGGCTAAACAAGTGT34335549.23568094544772No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34270889.21828849640126No Hit
NNNGAATCCACACCACCCCACCCTCCTCCTGTCCCGTGGTTTCATCATAT33917299.123178752226542No Hit
TTGATGCCCAACATTGGTTATGGAAGCAACAAAAAAACAAAGCACATGCT33909249.121013438047388No Hit
NNATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCAGAC33643279.04947199553445No Hit
GNNAATCGCTTGAACCCGGGAGGTGGAGGCTGCAGTGAGCAGACATCGCA33632109.04646745399642No Hit
TTCTTTTTTTCCTTTCTTTTTTTAAAATTGTAAAAATAAAATAAAGAAAA33615759.042069579856154No Hit
NNNGGGTCCAGTCTGTCTTAGNCNGNTTGGTCCACACCCTGGTTTNTNAG33371088.976257477966314No Hit
NNNGGAAAATTACGGGCAGAAGGAGGCTGAGAAAGTGGCCCGGGTGAAGG33365528.974761931775497No Hit
NNGCAGTGGTATCAACGCAGAGTACATGGGTAATGGGAACATTATGATCC33267228.948320890308331Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)