FastQCFastQC Report
Thu 16 May 2024
EGAF00006837123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006837123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80991633
Total Bases12.2 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14059731.7359484528482096Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3015450.3723162366660764Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1344190.16596652644354015No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1283750.15850402719994547Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)