FastQCFastQC Report
Fri 17 May 2024
EGAF00006837133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006837133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119503985
Total Bases18 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45491593.8067006719483034Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12782351.0696170508456266Clontech SMART CDS Primer II A (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4314520.36103565918743213Clontech SMART CDS Primer II A (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4312650.36087917904997063Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3240540.2711658527537806No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3201630.2679098943855303Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2999870.25102677538326446Clontech SMART CDS Primer II A (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)