FastQCFastQC Report
Sun 12 May 2024
EGAF00006837263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006837263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36961467
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10275672.780103397952251Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2266930.6133225177453049Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1013650.2742450671668416Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG749580.20280039209482678No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT464440.12565518571002607Clontech SMART CDS Primer II A (100% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT420320.11371843006123107Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380390.1029152874262269Clontech SMART CDS Primer II A (100% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)