FastQCFastQC Report
Sun 19 May 2024
EGAF00006837581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006837581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149395940
Total Bases22.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19826661.327121741059362Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3627840.24283390833780358Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2423480.1622185984438399No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1870110.12517810055614628Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)