FastQCFastQC Report
Wed 14 Sep 2022
EGAF00006837985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006837985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42787748
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT700247116.36559839512937No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT18862494.408385783706121No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT8784352.053005921227731No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG4151030.9701445376372695No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC3599870.8413319625982654No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT3454230.8072941815026115No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC2444970.5714182480461463No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA2396260.5600341480930476No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC1757770.410811524831828No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1404500.32824817048095173Clontech SMART CDS Primer II A (100% over 26bp)
GGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTG1313120.30689158962046803No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA1095430.25601487603413947No Hit
GCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCC1015210.2372665184435507No Hit
GCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAG1007450.23545291516627612No Hit
GGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGC1004760.2348242305250559No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTG968880.2264386524852862No Hit
GTCTCAGGGACCCCCCAGGCTTGACCAGGCCTCCCCCAGACTCCACCAGC877570.20509843144817996No Hit
GCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC851820.19908035356289377No Hit
GCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACT848930.19840492656916647No Hit
GTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGT842760.19696292499432316No Hit
GCCCTGCTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTT810750.1894818114755654No Hit
GCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAACAC801780.18738541696562297No Hit
GTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCC794970.18579383986275697No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTTGA778200.18187449360503852No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCT759210.17743630723449152No Hit
GCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAG755830.17664636147712193No Hit
GGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCC686360.16041040533378853No Hit
GATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGT680930.1591413504632214No Hit
GGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCA650300.1519827591767625No Hit
GTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGCC649060.1516929565912186No Hit
GTCCCAGGGCCGAAAGTGAATGAGGGTGAGCTACCATACTGCTGACAGTA648140.15147794176968604No Hit
GCTCAGGGATCCCTGAGGGCCGGTCGCTATTATCATAGACGACCAGCACA647760.15138913129992257No Hit
GATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTGGGAGCCT647250.15126993830102953No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA642540.15016915589948787No Hit
GAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGG641950.150031265959592No Hit
GCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGAC638980.14933714202486187No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG633190.147983950919782No Hit
GTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTCAA630930.14745576233645202No Hit
GACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTAT624970.1460628402317411No Hit
GGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACT621290.14520278094561087No Hit
GGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGT621200.14518174688698268No Hit
GTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGAT611300.14286800043788236No Hit
GGCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGG610680.14272309914511042No Hit
GCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTG610120.14259222055809062No Hit
GGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAG608380.14218556209127903No Hit
GCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGA599100.14001671693495063No Hit
GTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTGAATGAGGGTGAGCTA586620.137099994138509No Hit
GTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTGG581890.1359945375017166No Hit
GGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTGGGAGCC552570.12914210862417905No Hit
GGTCGCTATTATCATAGACGACCAGCACAGGGGCCTGGCCTGGCTTCTGC552290.12907666933066914No Hit
GTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTT548130.12810442839852193No Hit
GGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGA546850.12780527734247663No Hit
CCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGG544380.12722800928901423No Hit
GCCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGC541150.12647312029602495No Hit
GGAGAGTCTCAGGGACCCCCCAGGCTTGACCAGGCCTCCCCCAGACTCCA539100.12599401118282738No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC535690.1251970540725817No Hit
GCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCC526690.12309364820976322No Hit
GCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTG525880.12290434168210956No Hit
GCTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCT516910.12080794717216714No Hit
GCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTATAGCTACTGAAG516440.12069810264377549No Hit
GGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAAC514510.12024703894208219No Hit
GGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGCCTGGAGACTGCGTC511170.119466441655214No Hit
GCCTGGCGGACCCAGTTCATGCTATAGCTACTGAAGGTGAATCCAGAGGC510950.11941502506745622No Hit
GATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTG504070.11780708814121275No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG503980.11778605408258457No Hit
CCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGTTCATGCTATAG503590.11769490649519578No Hit
GAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCACCATAGATGAGGA501020.11709426726547983No Hit
GTTTCCCCCACAGGTAATCCTGGCCGTCTTTCCTGGGGCCACTGACACCG496050.11593271980567894No Hit
CTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGA491390.11484362299226404No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA489210.1143341313499369No Hit
GCCAGAGAATCGCTCAGGGATCCCTGAGGGCCGGTCGCTATTATCATAGA489090.11430608593843265No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC487660.11397187811800706No Hit
GGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGC486370.1136703899443364No Hit
GCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGC485790.11353483712206588No Hit
GCTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCC482700.11281266777583153No Hit
CTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTCTGACTGGCCCTGC477550.11160905219877429No Hit
CCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTGCTGGATGCAC474910.11099205314568086No Hit
GACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGG471750.11025352397606905No Hit
GGGTGAGCTACCATACTGCTGACAGTAATACACTGCAAAATCTTCAGGCT468340.10945656686582336No Hit
CTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCA467550.10927193457342041No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGA466530.10903354857563431No Hit
GGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGA464250.10850068575705364No Hit
GACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGA461840.10793744040934336No Hit
CCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTGCTGCTAACACTC461590.10787901246870951No Hit
GACAAAGACAGGGTGCCTGGAGACTGCGTCAACACAATTTCTCCGGTGGT456170.1066122947157677No Hit
CCAGTGGCCCTGCTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC451450.10550917519660068No Hit
CCCAGTTCATGCTATAGCTACTGAAGGTGAATCCAGAGGCTGCACAGGAG450320.1052450809049357No Hit
GAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCT449430.10503707743627919No Hit
ATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTAC448950.10492489579026221No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG445540.10412793868001652No Hit
AAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGA443660.10368856056645001No Hit
AGTAGCTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTT443650.10368622344882465No Hit
AGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCC441090.10308792133673407No Hit
GTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGAT439550.10272800522242956No Hit
GGCCCTGCTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTT435370.10175109005503165No Hit
CTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTG433030.10120420453069882No Hit
GCTGGTACCAGTGCACACTTTTACTTCCAATGTTGTTTCCCCCACAGGTA432970.1011901818249467No Hit
GCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGT432380.10105229188505083No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)