FastQCFastQC Report
Wed 14 Sep 2022
EGAF00006837987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006837987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38458218
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT551263414.334085890303081No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA17389964.521779974308742No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT14613493.799835447393844No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT11328372.9456305021725133No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC6596851.7153290877908072No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT5104791.3273599936429712No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC4467281.1615930826540117No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG2562870.6664037319669882No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG2495880.6489848281581846No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2247630.5844342553781353Clontech SMART CDS Primer II A (100% over 26bp)
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG2217720.5766569839507385No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA1897510.49339519579404323No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG1508420.39222306140133695No Hit
GAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTGA689220.17921267178838085No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT594200.15450533875490538No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC556940.1448169023328122No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG545870.1419384538305961No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC453990.11804759128465078No Hit
GCTTATTATTGTCATAAATGAGGAGTTTGGGGGCTGTTCCTGGGAGTTGC430580.11196046577093093No Hit
GGTCAGGAATCCCTGAGGGTCGCTTATTATTGTCATAAATGAGGAGTTTG417860.10865298022908915No Hit
GGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGCTAAGTAGCTG415120.10794051872086222No Hit
GGAGTTTGGGGGCTGTTCCTGGGAGTTGCTGGTACCAGGATACATAATTA408730.10627897527649356No Hit
GCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA391570.10181699006438624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)