FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006864024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006864024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14482905
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAA1516771.047282986389816TruSeq Adapter, Index 12 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1235050.8527640000400473TruSeq Adapter, Index 12 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTGTTCTGCGTGAAAAAAAAAAA452020.3121058931201993TruSeq Adapter, Index 12 (98% over 60bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAG232770.160720518431903TruSeq Adapter, Index 12 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAG193630.13369555348184636TruSeq Adapter, Index 12 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATGTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAA164520.11359599472619615TruSeq Adapter, Index 12 (98% over 60bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTTTGCGTGAAAAAAAAAAA163760.11307123812522418TruSeq Adapter, Index 12 (98% over 60bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTTTGCTTGAAAAAAAAAAA152900.10557274248501941TruSeq Adapter, Index 12 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTGTTCTGCTTGAAAAAAAAAAA147920.10213420581022938TruSeq Adapter, Index 12 (98% over 63bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)