FastQCFastQC Report
Thu 27 Oct 2022
EGAF00006864026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006864026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32231502
Sequences flagged as poor quality0
Sequence length75
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAA3307851.0262785767787055TruSeq Adapter, Index 7 (98% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTGTTCTGCGTGAAAAAAAAAAA1654920.5134479925881208TruSeq Adapter, Index 7 (98% over 60bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA743260.23060048520233403TruSeq Adapter, Index 7 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTTTGCGTGAAAAAAAAAAA665050.20633540441273882TruSeq Adapter, Index 7 (98% over 60bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTGTTTTGCGTGAAAAAAAAAAA653670.20280469709416582TruSeq Adapter, Index 7 (98% over 56bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGGGTTCTGCGTGAAAAAAAAAAA431610.1339093660605702TruSeq Adapter, Index 7 (98% over 52bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGGCTTCTGCGTGAAAAAAAAAAA417630.12957199450401038TruSeq Adapter, Index 7 (98% over 60bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)