FastQCFastQC Report
Thu 29 Sep 2022
EGAF00006871103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006871103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59999745
Sequences flagged as poor quality0
Sequence length150
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG4200900.7001529756501466No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC3829820.6383060461340294No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC2383960.39732835531217675No Hit
CCGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCC1812810.30213628407920734No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG1642290.27371616329369397No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA1556620.2594377692771861No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC1549310.2582194307659141No Hit
GCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAG1471560.24526104235943003No Hit
ACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGC1385860.23097764832167206No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC1347210.22453595427780565No Hit
CGCGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCA1213960.20232752655865455No Hit
CACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCT1192520.19875417803858997No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG1178960.1964941684335492No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA1147400.19123414607845418No Hit
CGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGGCG1127360.18789413188339382No Hit
CTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCATCC1071070.1785124253444744No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG1016980.16949738703056155No Hit
GCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGC956280.1593806773678788No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT927360.15456065688279175No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTC920510.15341898536402113No Hit
GGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGG842000.14033392975253478No Hit
GTCCGCGGGGCTCCGGGGGCGGGGAGCGGGGCGTGGGCGGGAGGAGGGGA832180.13869725613000522No Hit
GACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGACGGA831850.13864225589625423No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA824530.1374222507112322No Hit
GACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGGG800190.1333655668036589No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG799310.13321889951365626No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC783440.1305738882723585No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG776770.12946221688108842No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG769040.12817387807231514No Hit
CTTCAAGGCTCACCGCAGCGGCCCTCCTACTCGTCGCGGCGTAGCGTCCG759910.12665220493853765No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA759850.12664220489603747No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG697530.11625549408584987No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC695760.11596049283209453No Hit
CCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCC669060.11151047391951416No Hit
CTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAA656870.10947879861822746No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC615410.10256876925060264No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG608290.10138209754058122No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT607040.10117376332182744No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)