FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006871233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006871233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59999876
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTC2396280.3993808253870391No Hit
CGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGC1246150.2076920958969982No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC1125400.18756705430524556No Hit
CTCCACTTCGGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCA1075730.17928870386332132No Hit
GGCCTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCA976250.16270866959791716No Hit
GTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGC825240.13754028424992076No Hit
GCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGC797860.13297694148567907No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA797630.13293860807312335No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT687490.11458190346926717No Hit
CTTCAAAGTTCTCGTTTGAATATTTGCTACTACCACCAAGATCTGCACCT654860.10914355889668839No Hit
CTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGCCAT600490.10008187350253857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)