FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006975327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006975327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38012143
Sequences flagged as poor quality0
Sequence length65
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC6025101.5850461259182362No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4637801.2200838032204604No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCC2585070.6800642626226046No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC1419590.3734569766298101No Hit
CAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCCCT1314800.34588947010959104No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTCTCCAGCAACTGGAAG1270180.3341511158684213No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA1161630.30559445175190464No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGT1151260.3028663761472222No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT1087500.2860927888227717No Hit
ACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTT1045840.27513313311485754No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG972930.2559524202568637No Hit
CTCAGACCGTGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC972760.2559076977059673No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCTTTCCAAGGAATGTA927740.24406411393327654No Hit
GACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTT927620.24403254507382022No Hit
CCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTTA889930.23411729246625218No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCCT833180.2191878526817075No Hit
CTCAGTTCCGATGAGAACGACGGTATGAGTTCTCACTCATTTTGTTCAGCTTTTCCAAGGAATGT816720.2148576574596176No Hit
CTGGTTTTGTTGGATACTCGTCAGCTGTGAGCCGATCACTCTCAGAAACACACACTGACAGAATC785530.20665238473926606No Hit
CAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTTAA780390.205300185259221No Hit
CTCCTGTGTAACTGGTTTTGTTGGATACTCGTCAGCTGTGAGCCGATCACTCTCAGAAACACACA739120.1944431283445398No Hit
CCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCCC738730.19434052955130682No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCTTTCCAAGGAATGCA637640.16774639619765713No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTA620380.16320574191252515No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCT588490.1548163175120119No Hit
TGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGC554880.14597440612595822No Hit
TGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTG554860.14596914464938215No Hit
GCCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCC536380.14110754029311107No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCG528800.13911344067078774No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGC516040.13575661861526725No Hit
CTCATATCTCATAATCTTCGCTCACAGGACGCTGGACATTCAAAGAAAGAAAAAGAAGTACAGAA504380.1326891777714295No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG501820.13201570876969498No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAA495690.1304030661991354No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTC486840.1280748628142328No Hit
GCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCCCTAC486480.1279801562358639No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCA480650.12644643581394502No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAA457950.12047465990012718No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGG456160.1200037577465706No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCA452760.11910930672864195No Hit
TCTAAATCGATCAATAAATAGGACACTTCTGCCAAAGGAACTCGTGCACAGCACCACAGAACATG449880.11835165410169061No Hit
CTCAGTTTGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCTTTCCAAGGAATGTA434070.11419245686832233No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG426950.11231937120724816No Hit
CTCAGTTCAACGAAGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCTTTCCAAGGAATGCA417750.10989909198226473No Hit
CCCGTCCCTGCTGACACCAACCATTCTTACCGGTAGCCTGCCAACACACAAGCAGTTCACAGTAG411890.10835747934548179No Hit
CAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGT411840.10834432565404166No Hit
CTCAGTTCCGATGAGAATGACGGTATGAGTTTTCACTCATTTTGTTCAGCTTTTCCAAGGAATGT410740.10805494444235886No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGT409690.10777871692211619No Hit
GTTTTGTTGGATACTCGTCAGCTGTGAGCCGATCACTCTCAGAAACACACACTGACAGAATCCCA394400.1037563180797252No Hit
GCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGG382140.10053103293860596No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)