FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006975329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006975329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38313962
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC27428737.158938561352647No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA5802921.5145705891758205No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG4236481.1057274630068277No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT3495050.9122131509134972No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG2518830.6574183061516844No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATAGCTGAC2333340.6090051454349723No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCT2232630.5827196884519539No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGT2095000.5467980575853784No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC2005670.5234827972111054No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGG1993300.5202542091574868No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCC1676370.4375350166083059No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTC1627750.4248451256489736No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1225680.3199042688406905No Hit
ACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTT1155820.3016707068822587No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTA1122620.29300545842792247No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCCT1053640.2750015777538225No Hit
GACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTT1008180.26313645140640896No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTCTCCAGCAACTGGAAG1002320.2616069828539267No Hit
CCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTTA988920.25810956329705603No Hit
AGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC976670.2549122954185735No Hit
CAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTTAA851740.22230538308724118No Hit
CAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCCCT844970.22053840320664306No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTT838940.21896456440605125No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCA755770.19725707302210094No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTC751070.1960303661626015No Hit
TCTAAATCGATCAATAAATAGGACACTTCTGCCAAAGGAACTCGTGCACAGCACCACAGAACATG741110.1934307916263006No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAA722030.1884508837796519No Hit
GCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACC702970.18347619596219258No Hit
GTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA684610.17868420916636082No Hit
CAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTC673450.17577143287869837No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCA648200.16918114602713236No Hit
CGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCA640480.1671662147600397No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAA628770.16410988766966986No Hit
CACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAA627520.16378363584533492No Hit
TGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGT599940.15658521559320857No Hit
GTCCAAGGCATGAACCAGGCATGGGCACGGATCAAAAGCCAAGCTGGGATGAACAAGGAGTTGCT594500.15516536765370284No Hit
CCCAGACTAGGATCAACTCCACAGGAGTCATTCCACTCTCAGAGGTGCAAAACACTGCCCAAAGG583920.15240397221253182No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAA562930.1469255515782993No Hit
GACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCA562630.14684725114045893No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGT559470.14602248652854016No Hit
ACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG555080.14487669012147583No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATAGCTGA549530.14342813202142865No Hit
CGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAG533300.1391920783342636No Hit
CCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAG523530.13664209407526165No Hit
GCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCCTATG517840.13515699577088897No Hit
CTGGTTTTGTTGGATACTCGTCAGCTGTGAGCCGATCACTCTCAGAAACACACACTGACAGAATC517010.13494036455953054No Hit
GCCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGC516190.13472634336276681No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGC513950.13414170009355858No Hit
CCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAG510790.1333169354816398No Hit
CTCTTACATGCCAATGACTCAAGCACCTTTGACTATACAACCAAAAAAGTTGCTGGATGTTTCTT508910.13282625273784007No Hit
CTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTTCACGCTCAGGAGAAAACGCTACCT492170.1284570883063464No Hit
CTCAGTTCCGATGAGAACGACGGTATGAGTTCTCACTCATTTTGTTCAGCTTTTCCAAGGAATGT482840.1260219446895103No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTT477900.1247325974797386No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCGGCTT470650.12284033689859586No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAA460240.1201233117055344No Hit
GCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGC457420.11938728758983475No Hit
ACGAGGCCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTAT453790.11843985229196605No Hit
TGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGC453460.11835372181034161No Hit
CCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCCC449350.11728100581192831No Hit
GGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAG440810.11505205334807192No Hit
CGAGGCCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATG428760.11190698576148297No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAA422860.11036707715062202No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCG419990.10961800296194896No Hit
CGACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTTATTTCCAA415540.10845654646731653No Hit
GCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTAGGAATATGCAGGCGCA412740.10772574238080625No Hit
CACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAA411250.10733685020619897No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCTTTCCAAGGAATGTA408370.10658516600293125No Hit
CTCCTGTGTAACTGGTTTTGTTGGATACTCGTCAGCTGTGAGCCGATCACTCTCAGAAACACACA405340.10579433158074333No Hit
CTCTAACCTGGTGACTGACTGTGCCATAAGGCCCACAGCCAGTGAATAAGGTCAGCAGTTACTCT404980.10570037105533488No Hit
ACCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGC404130.1054785198147871No Hit
CAAGGCATGAACCAGGCATGGGCACGGATCAAAAGCCAAGCTGGGATGAACAAGGAGTTGCTTTG398280.10395166127689953No Hit
CTAAATCGATCAATAAATAGGACACTTCTGCCAAAGGAACTCGTGCACAGCACCACAGAACATGG394420.1029441956433532No Hit
CAAGACCTGAAACTGAAAGCCACCAGTCAGTCAGTCATGTGTCGCTGGAAATGCTATTGCACCGG393880.1028032548552405No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)