FastQCFastQC Report
Mon 19 Sep 2022
EGAF00006975330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006975330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14118726
Sequences flagged as poor quality0
Sequence length65
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGAC6977954.942336865238407No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGA1661641.1769050550311693No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTG1063230.7530636971069486No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTT724140.5128933021293848No Hit
TCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACG706630.5004913332831872No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGT590420.41818220709148973No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCT563010.3987682741346492No Hit
CAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGG531580.376507058781366No Hit
CCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCC412640.2922643303652185No Hit
CCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCA412530.29218641965287806No Hit
CTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAA403520.28580482403298996No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTC374640.2653497206475995No Hit
CCTGAACATGCCCTGTTCCTACTCTCTCACTCCAGTTACCACAATTTCTCTCCAGCAACTGGAAG330300.23394462078235673No Hit
AGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGC315420.22340542624029958No Hit
CCCAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCC305190.21615972999263533No Hit
GTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTA277930.19685203891625916No Hit
GGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTT272440.1929635860912663No Hit
CTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAA270540.19161785560538536No Hit
CCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAG250880.177693086472533No Hit
CGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTT247730.175462006982783No Hit
ACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTT235000.16644561272738065No Hit
GTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCA233800.16559567768366637No Hit
CCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGTACATCATGGGGCAGAGCCCTAAGCTCCCCT231090.16367624104327827No Hit
CTCAGTTCCGATGAGAACGACGGTATGAGTTCTCACTCATTTTGTTCAGCTTTTCCAAGGAATGT220690.1563101373310878No Hit
CCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTTA216180.1531157981251283No Hit
GACCAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTT215300.15249251242640446No Hit
CTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTC211670.14992145891916878No Hit
CAAGCGTCCCTGGCTGCTACAGGAGAATAGCAGACAGGTATAGTTAAGAAACAACTTTATTTTAA209140.14812951253533782No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGATCATCAATGGCTGAC194310.13762573195343547No Hit
CTCCAAGACCTGAAACTGAAAGCCACCAGTCAGTCAGTCATGTGTCGCTGGAAATGCTATTGCAC182050.12894222892348786No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAG180360.1277452370702569No Hit
CAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGGTGCTCTACACGTTCAGAGAAACTTCTC177670.12583996601393072No Hit
CCAAGACCTGAAACTGAAAGCCACCAGTCAGTCAGTCATGTGTCGCTGGAAATGCTATTGCACCG171490.12146280053880215No Hit
CAAGACCTGAAACTGAAAGCCACCAGTCAGTCAGTCATGTGTCGCTGGAAATGCTATTGCACCGG164590.11657567403744501No Hit
CCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAA164470.1164906805330736No Hit
TCTAAATCGATCAATAAATAGGACACTTCTGCCAAAGGAACTCGTGCACAGCACCACAGAACATG155860.1103923965944236No Hit
ACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAG154800.10964162063914265No Hit
CAGCTTTATTTCCAACGTTGTGGAAAGGGACTTGTACATCATGGGGCAGAGCCCTATGCTCCCCT153850.10896875539620218No Hit
CTCAGTTCGACGAGGATGACGGTATAGAGTTTTCACTCATTTTGTTCAGCTTTCCAAGGAATGTA153740.1088908446838617No Hit
CCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAA146440.10372040650126646No Hit
TGTAGGAATATGCAGGCGCAGACACGAGGCCCAGCTTCATCTTCAACGTTGTGGAAAGGGACTGT145460.10302629288223314No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCCAAGCAACGCCAGAAAGCCGGCTTCACGCTCAGGA145340.1029412993778617No Hit
GTCCAAGGCATGAACCAGGCATGGGCACGGATCAAAAGCCAAGCTGGGATGAACAAGGAGTTGCT144210.1021409438783641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)