FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5943792
Sequences flagged as poor quality0
Sequence length35-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT947251.5936795904028942No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA805991.3560198607219096No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT523660.8810200626132273No Hit
TGGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCT520870.8763260894728483No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA357690.6017875457283834No Hit
GGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTC302850.5095232134637282No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA294350.49522257844823636No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT275870.4641313154969084No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT259870.4372124731148062No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT235600.39637995407645493No Hit
TTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG224380.3775031158560057No Hit
TCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCA224280.3773348730911176No Hit
GTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTA219950.3700499613714612No Hit
GGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCATAG216830.36480078710695124No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG214930.3616041745740766No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC212430.35739810545187317No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA210000.35330980626509134No Hit
TGGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTAC202200.3401868706038166No Hit
GGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACT199740.33604809858756834No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA195180.3283762285086692No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA190490.3204856428354155No Hit
GGTCCTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTAT173430.29178342714549904No Hit
CTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTA170900.28752688519382913No Hit
GGGTTAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA148990.2506648954068379No Hit
CTCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGC141140.237457838363119No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA136480.2296177255193318No Hit
GAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATAC129530.21792485335960615No Hit
TTCGTTATTGAGTTTAGGTCCTAACAATGAATTTTCACATATAAGTTGGA123870.20840231286693744No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT122500.20609738698796998No Hit
TGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTA120910.20342232702624855No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG119760.20148753523003496No Hit
ATGGCTAGTAGTTCTCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGG109270.18383886919326922No Hit
TCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTT108640.18277893977447393No Hit
TTGGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGC106970.17996928560084202No Hit
GGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAG104320.17551085233130634No Hit
TGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA102890.17310498079340597No Hit
GGTGTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAAT95960.16144575718665793No Hit
GTTGGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGC94970.15978015381426539No Hit
TGTTACTCATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAA94780.1594604925609779No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG92160.15505253212090866No Hit
GGTGGTTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT91020.1531345646011839No Hit
TGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCATTCCGGTGT84570.14228290626589893No Hit
CGTCTTATTATTATATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGA83000.13964149485715519No Hit
AGGTCCTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTA82940.13954054919822226No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT80070.1347119818459327No Hit
TGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTAT79160.13318097268545062No Hit
GGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTCC78450.13198644905474485No Hit
TTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGT71930.1210170207840382No Hit
GGGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCT71240.11985614570631004No Hit
TCCCATTTCATTGGCTACACCTTGACCTAACGTTTTTATGTTTGATTCTT70990.1194355387940897No Hit
GCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGG70220.11814006950445102No Hit
CGTAGGACTTTAATCGTTGAACAAACGAACCATTAATAGCTTCTACACCA69440.11682777593832354No Hit
AGATGGATATAAAGTACCGCCAAGTCCTTTGAGTTTTAAGCTATGGCTAG68900.11591926500792761No Hit
GCCGTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTG67200.11305913800482924No Hit
CCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCA65670.1104850237020407No Hit
GGCGGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTT64800.1090213116475139No Hit
CCTCTCATAAACGGATGTCTAGAAATTAATTATGTTAAGTTTAATTTAAT64650.10876894750018169No Hit
CCTTAGTCCTTTAGTTTCATAAAGGGTATAGTAATGTTCTTTTATAAGAA64540.10858388045880474No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG63560.10693510136290099No Hit
AGGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCT63560.10693510136290099No Hit
CCTCCTTAGTCCTTTAGTTTCATAAAGGGTATAGTAATGTTCTTTTATAA63060.10609388753846029No Hit
GTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACTT62640.10538726792593012No Hit
TAGTCCTTTAGTTTCATAAAGGGTATAGTAATGTTCTTTTATAAGAAAAT61940.10420956857171314No Hit
TGGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTT61010.10264491085825345No Hit
CTATTCTTAATTTACTACTAAATCCTCCTTAGTCCTTTAGTTTCATAAAG60350.10153450860999173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)