FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8734606
Sequences flagged as poor quality0
Sequence length35-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1786862.045724787128349No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN839430.9610393416715076No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC278250.3185604479469366No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG253600.2903393696292655No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA234670.26866695532689167No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG208530.2387400187255155No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG154740.17715738981243115No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG149140.17074611035689533No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104460.11959325927237015No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC90910.10408025273263613No Hit
GTCTGTCTGTCTCTCCCTCTCTCTCCCTCCCTTTCTGTTTCTCTCTCTCT90810.10396576559950158No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG90610.10373679133323242No Hit
GTCTGTTTCTCTCTCTTTCTGTTTCTCTCTGTCTCTGTCCATCTCTGTCT87930.100668536165226No Hit
GTCTGTCTCTTTCTCTGTCTGTCTGTCTCTCTCTTTCTTTTTCTCTGTCT87440.1001075492128666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)