FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10156283
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2803922.7607737988395953No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2656832.615947192491584No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT643920.6340114784119347No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC443890.43705950296973806No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG364890.3592751403244671No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG260050.2560483988088949No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG244130.24037337281759477No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG178590.17584189018758142No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC176540.1738234352075459No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA167720.1651391557324663No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG152790.1504388958046955No Hit
GGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG122590.12070360780612355No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG117070.11526854854280844No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG112810.1110741006330761No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG111770.11005010396027759No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG105720.10409320023870938No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)