FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11142264
Sequences flagged as poor quality0
Sequence length35-101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG837160.7513374301667956No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA527140.4730995424269251No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA517030.4640259825112742No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG484330.43467826646361996No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG463380.4158759835523553No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA429700.38564873350694256No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG384040.34466962908076854No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG370900.33287669364143585No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT359500.3226453797899601No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC332290.2982248490970955No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN264510.23739340586437369No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA263990.236926714355359No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC262680.2357510107461105No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG254750.22863396523363655No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG253010.22707234364577972No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG248250.222800321370953No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG230390.2067712630036409No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC218990.1965399491521651No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG216930.19469113278953004No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG214350.19237562491788024No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG200670.1800980482961093No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG199700.17922748913506267No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG193390.17356436716990373No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG189900.17043214915747823No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT184610.16568446053692498No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA182900.16414976345920362No Hit
TCTGGGTGGTATTACACAGGGCCTGATGGGTTTTGGGAACTGCTCCTACG176410.15832509443323187No Hit
TGGCCAAATTGGTGGGGCTTCGGCCCGCGTTTTGGAGACCTGCTAAAAGC173740.1559288130311757No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG157920.1417306213530751No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG152080.1364893167133717No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC142660.12803502053083646No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC142310.12772090124592272No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG135730.1218154586895446No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT131860.11834219688206993No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG130060.11672672627394216No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA129530.11625105992821567No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG128380.11521895370635628No Hit
GTTTCGGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT127800.11469841317707065No Hit
GCTGGCAATCATCCCAGGTGGGCTGGTGGGTGGTGAGGACGGATTCAATC122580.11001354841350018No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121890.10939428468038452No Hit
CTGTGTAGTGCCTCATCAAACAGGGTGGGACTGTTTTTGAAACCCTGTGG119080.10687235556436286No Hit
TCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA118780.10660311046300824No Hit
TCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA114940.10315677316566903No Hit
GGGTGCTGGATCCGGAAGTCTGCTAGGTCTCTGTGTAGTGCCTCATCAAA111740.10028482541788634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)