FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9235593
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG457510.4953769617175638No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG395750.4285052405405912No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT365450.3956973851056451No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA277430.30039218921838584No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN229640.24864673010168378No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG188570.20417746862599942No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG184500.19977060487615683No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG170600.18472013654131358No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG159180.17235493162160784No Hit
TGGCCAAATTGGTGGGGCTTCGGCCCGCGTTTTGGAGACCTGCTAAAAGC157600.17064415896196378No Hit
TCTGGGTGGTATTACACAGGGCCTGATGGGTTTTGGGAACTGCTCCTACG156370.16931235492945607No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC155840.16873848815122103No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154290.1670601985167601No Hit
TCTGGAGTTTCTCTTTTATTAAAGATCCTTTCGGCTTCTCTCACTAAATC152380.16499211258010177No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA149250.16160305028599678No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC137550.14893467046458198No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA132430.14339090083333036No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA127160.13768471607616317No Hit
GCTGGCAATCATCCCAGGTGGGCTGGTGGGTGGTGAGGACGGATTCAATC126150.1365911208949983No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC125250.13561663013950484No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG123750.1339924788803491No Hit
GTTTCGGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT117380.1270952498664677No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG117010.12669462588920927No Hit
CTGTGTAGTGCCTCATCAAACAGGGTGGGACTGTTTTTGAAACCCTGTGG113380.12276417984205237No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT110540.1196891201247175No Hit
GTTTTAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT105230.11393962466730616No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG104520.11317085973797245No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG102100.11055056237320117No Hit
GGGTGCTGGATCCGGAAGTCTGCTAGGTCTCTGTGTAGTGCCTCATCAAA100900.10925124136587655No Hit
GGTGGTAAACATAACTAGGGGAATGGTAGGTCACCCTTGGCCAAAGCTCG97480.10554817649500146No Hit
CCTGGTTTCTTAACGGGTAGCAGGGGTGTATTCCAGGGGGACTGGCAGGG94640.10247311677766657No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG94560.10238649537717827No Hit
GGGCTTTTAGTTTAGTTAACAAGTCTCTTCCTAGCAAGGGGTAGGGGCAG94260.10206166512534713No Hit
TTGGCCAAATTGGTGGGGCTTCGGCCCGCGTTTTGGAGACCTGCTAAAAG94160.10195338837473673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)