FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747015
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG471410.5389381406113972No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT391440.44751266574940135No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN302570.34591229122163386No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG176630.20193174471519715No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA166660.1905335705952259No Hit
TCTGGAGTTTCTCTTTTATTAAAGATCCTTTCGGCTTCTCTCACTAAATC164370.18791553461380825No Hit
TGGCCAAATTGGTGGGGCTTCGGCCCGCGTTTTGGAGACCTGCTAAAAGC161690.18485163224254217No Hit
TCTGGGTGGTATTACACAGGGCCTGATGGGTTTTGGGAACTGCTCCTACG156220.1785980703131297No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG146430.16740568068078082No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139480.15946011296425125No Hit
GCTGGCAATCATCCCAGGTGGGCTGGTGGGTGGTGAGGACGGATTCAATC137430.1571164562996634No Hit
CTGTGTAGTGCCTCATCAAACAGGGTGGGACTGTTTTTGAAACCCTGTGG121710.13914461104731157No Hit
GTTTCGGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT121530.13893882655968923No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG110140.12591724148180836No Hit
GTTTTAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT108520.12406518109320723No Hit
GGTGGTAAACATAACTAGGGGAATGGTAGGTCACCCTTGGCCAAAGCTCG101820.11640542516504201No Hit
TTGGCCAAATTGGTGGGGCTTCGGCCCGCGTTTTGGAGACCTGCTAAAAG101590.11614247831974679No Hit
GGGTGCTGGATCCGGAAGTCTGCTAGGTCTCTGTGTAGTGCCTCATCAAA100190.1145419323049063No Hit
CCTGGTTTCTTAACGGGTAGCAGGGGTGTATTCCAGGGGGACTGGCAGGG99750.114038903557385No Hit
CCTTTTTGATGCCCCAGGCAGTGGATTATACTGAGTCTCTTTGGCAGAAA97280.111215083088345No Hit
TGGGGTATTGTTTTATGGACACAGGGGTGGAGGTTGCCTTCAGAGGTATG96370.11017472817869867No Hit
GGGCTTTTAGTTTAGTTAACAAGTCTCTTCCTAGCAAGGGGTAGGGGCAG93390.10676785166139535No Hit
TGCTGTTTCTCTCTTGGTTGGAAAGGCTTCCACCCAACCAGAGAAGGTGT91460.10456138465522238No Hit
GTGGTCCAGCGATACCGCTTTCCTCCAGTAGCCCCTTGGACCCAAGCCGA90490.1034524349163686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)