FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747015
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN298470.34122497789245815No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC199420.22798634734249343No Hit
TTTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG163050.18640644837124437No Hit
TTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT161950.1851488765024411No Hit
GTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT112280.1283637903902074No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT106830.12213309340386405No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT103210.11799453870834793No Hit
GTTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG99170.11337582020837966No Hit
TTTGGCGCCAGTCCTTCGATAGACTGAGTCGCCCGGGTACCCGTGTATCC96040.1097974566180577No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC91400.10449278982601494No Hit
GGGGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG89090.10185188890152813No Hit
GTGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT88330.10098302106490042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)