FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12031221
Sequences flagged as poor quality0
Sequence length35-101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG719520.5980440389217354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN686440.5705489077126918No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG657980.5468937857595667No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA601990.5003565307295078No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA515570.4285267472021335No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT452440.376054932412928No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG386000.3208319421611489No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG339930.2825399018104646No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG334460.27799339734512396No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG332370.27625625030077994No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG330890.27502611746555067No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG326780.27161000533528556No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323010.26847649128878937No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG307620.25568477214407415No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA292800.2433668203750891No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC292220.24288474129101276No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG283370.23552887940467554No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA247050.20534075469148144No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG229500.19075370654399915No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC216130.17964095248520495No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG206950.17201080422344497No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG196430.16326688704330178No Hit
TCTGGAGTTTCTCTTTTATTAAAGATCCTTTCGGCTTCTCTCACTAAATC193060.1604658413306513No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG181750.1510652991911627No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG174450.14499775209847776No Hit
TCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA169990.14129073017609767No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG166800.13863929521367782No Hit
TCTGGGTGGTATTACACAGGGCCTGATGGGTTTTGGGAACTGCTCCTACG161150.1339431799981066No Hit
TGGCCAAATTGGTGGGGCTTCGGCCCGCGTTTTGGAGACCTGCTAAAAGC154260.12821641294761355No Hit
CCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG151940.1262880966113082No Hit
TCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA150680.12524082135969408No Hit
GTTTCGGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT137150.11399507996736158No Hit
GCTGGCAATCATCCCAGGTGGGCTGGTGGGTGGTGAGGACGGATTCAATC129340.1075036357490233No Hit
CCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG128610.10689688103975481No Hit
GTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG126820.10540908524579509No Hit
CCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG125490.10430362803575796No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC123290.10247505219960634No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG122720.10200128482387615No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC120850.10044699536314726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)