FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9182957
Sequences flagged as poor quality0
Sequence length35-101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1981532.1578343446451944No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC773540.8423648286712003No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG629370.6853674693238789No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT624860.6804561972793731No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA576110.627368722297186No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA565840.6161849609009385No Hit
TGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT507870.5530571470605818No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT367310.39999098329655686No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG344610.37527127699715895No Hit
GGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCTC339680.3699026359374219No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA319240.3476440105295059No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT295690.32199867646118785No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG292690.31873175492382244No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG286150.31160986597236595No Hit
TGGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTAC284980.3103357665727935No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG284830.31017242049592525No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG282670.30782023698902217No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT282670.30782023698902217No Hit
GGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTACT280670.30564228929744525No Hit
GTTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGC280660.3056313995589874No Hit
TTTGGCTATAATTTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG265650.2892859021337027No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA262990.2863892317039054No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA255340.27805858178362375No Hit
TCATTGCGCCACGGCGGCTTTCGGACGAGCCCCTGACTCGCGCACGCGTT239180.26046076443568233No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA227000.24719706299397895No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG226950.24714261430168952No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGTTGTTTTGTTAATTATT221430.24113147867293724No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT206200.22454640700157913No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG194500.21180541300585423No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG187370.20404102948538252No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA184800.2012423667017062No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA176890.19262858358151955No Hit
ACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGG172430.18777176022930303No Hit
GTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTA171080.18630164553748862No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC166700.18153194009293522No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG164690.1793431026629004No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG160290.17455161774143121No Hit
CCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG159440.17362598997251102No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA158980.17312506200344835No Hit
CCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG156030.1699125891583724No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT151690.1651864426676505No Hit
CCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG151510.1649904273754086No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA148300.16149482133042764No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG145570.15852192273142518No Hit
CCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGA143650.15643109294751137No Hit
TCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGG139980.15243455893346772No Hit
TCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA139690.15211875651818907No Hit
TCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA136300.14842713518096623No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA132660.14446327038229625No Hit
CCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCG128980.14045584662979474No Hit
TGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA125580.13675333555411398No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG124780.13588215647748322No Hit
CCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG123410.13439026230875306No Hit
GGTCCTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTAT120910.1316678276942819No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN118040.12854247275686906No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA116090.12641897375758157No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA115830.12613584055767657No Hit
GGGGTTCTTGTTTGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAG113390.12347874437395276No Hit
GCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCG112550.12256400634349045No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC110770.120625632897987No Hit
ACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA110060.11985246146747719No Hit
GTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG109780.11954754879065642No Hit
TGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTA109280.1190030618677622No Hit
GGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCATAG108880.11856747232944682No Hit
TCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCA108570.11822989043725239No Hit
GCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATTTGGG108330.11796853671426318No Hit
GCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA105740.11514809445367108No Hit
TGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCC104510.11380865662335127No Hit
TCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG102960.11212074716237919No Hit
TGCCGAGTTCCTTTTATCTTTTTGGATCTTTCCTTTAGGCATTCCGGTGT102450.11156537050102708No Hit
GAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATAC100520.10946365097865535No Hit
CCCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA100370.10930030490178709No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT98780.10756883648698344No Hit
CTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG98110.10683922401030518No Hit
TCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG97930.10664320871806325No Hit
TTCGTTATTGAGTTTAGGTCCTAACAATGAATTTTCACATATAAGTTGGA97900.10661053950268962No Hit
GGGGACAGTCCGCCCCGCCCCCCACCGGGCCCCGAGAGAGGCGACGGAGG97170.10581558859526403No Hit
CATTGCGCCACGGCGGCTTTCGGACGAGCCCCTGACTCGCGCACGCGTTA96300.10486818134942807No Hit
GGCTGCTTTTAGGCCTACAATGGTTAAAAGTTGTTTTGTTAATTATTCAC96080.10462860710335463No Hit
TTGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGC94750.10318027188845598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)