FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11631538
Sequences flagged as poor quality0
Sequence length35-101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1974891.697875207904578No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1114430.9581106127151887No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC586710.5044130879338571No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT417160.3586456064537639No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417000.3585080494084273No Hit
TGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT298540.25666425196736664No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT249610.21459758804037782No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG241010.20720389685353735No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT221620.19053370242181214No Hit
GGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCTC218740.18805767560575393No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA210110.18063819247291288No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA206710.1777151052595108No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT202830.17437934691009907No Hit
TTTGGCTATAATTTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG201520.17325309860140595No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA194780.16745850806660306No Hit
GTTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGC192340.1653607631252204No Hit
GGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTACT183040.15736525986503247No Hit
TGGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTAC178890.15379737400161528No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA171780.14768468279947158No Hit
CCCCTGGTCCGCACCAGTTCTAAGTCGGCTGCTAGGCGCCGGCCGAGGCG170070.14621454187743702No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA168710.1450453069920762No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG165460.1422511795086772No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT146580.1260194481589623No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGTTGTTTTGTTAATTATT139240.11970901870414728No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC133600.11486013285603332No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA127060.10923748862790114No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG126210.10850671682455064No Hit
GTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTA125020.10748363629985991No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG118590.10195556254039663No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)