FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7965871
Sequences flagged as poor quality0
Sequence length35-101
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1735272.1783807445538597No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG1113041.397260889612699No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA1065991.3381964131731483No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA1001811.257627696958688No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG755010.94780595869554No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG652330.8189060555964313No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC611080.7671226410771653No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG606620.7615237555315671No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG509820.6400053427930229No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG476710.5984405220722254No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG454070.5700192734730452No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG385780.48429104613921065No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA370460.4650589998256311No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG339100.425691051235954No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG306780.3851179613629194No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC298540.37477383201410114No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG287880.3613917423468193No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA275720.34612661942429146No Hit
CCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG254180.3190862618789584No Hit
TCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA244680.30716038459573347No Hit
TCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA233180.2927237963055139No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC233030.29253549297998926No Hit
CCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG223360.2803962052611698No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT217900.2735419642120742No Hit
ACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA209350.26280867465717184No Hit
GCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA203440.25538952363150247No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA202820.2546112032193341No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG198160.24876124657303642No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC198080.24866081813275662No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG194230.24382769944429178No Hit
CCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG191410.24028759692442922No Hit
GTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG189140.2374379399314902No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT187340.235178300025195No Hit
CCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG187280.23510297869498514No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG179390.22519822377239102No Hit
TCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG178560.22415627870448818No Hit
TCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGG177090.22231090611434706No Hit
TCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG177020.22222303122910225No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG175630.22047808707924094No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG169830.21319702515895625No Hit
CCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGA167300.21002097573510795No Hit
ATCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG167230.20993310084986314No Hit
CTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG158060.19842149088279237No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG155930.195747583660343No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG150980.18953357391803108No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG150490.18891844972131736No Hit
TCCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA141560.17770812507508596No Hit
ACCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG139880.1755991278292104No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG137780.17296288127186596No Hit
CCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA132230.1659956582274556No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA128870.16177766373570449No Hit
TGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT127150.15961845226968902No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG125750.15786095456479274No Hit
ACCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA123370.15487320846646901No Hit
GCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG122270.15349231741262193No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC120360.15109458840094198No Hit
CCCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA118160.14833280629324777No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC118000.1481319494126882No Hit
TTCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA116750.1465627550333165No Hit
CCCCATACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG115770.14533250663988911No Hit
CCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGG109930.13800123049946453No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG109110.1369718389865967No Hit
CCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG108140.13575414414820428No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA107440.1348753952957561No Hit
GCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA105780.13279150515995047No Hit
TCTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA103730.1302180263777809No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC103580.1300297230522563No Hit
GGTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCG102150.12823456468225508No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT97710.12266078624672681No Hit
CTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA96030.12055178900085126No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT95220.11953495104301838No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG90850.11404904749273495No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG90750.1139235119423852No Hit
CCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG90620.11376031572693054No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA90620.11376031572693054No Hit
GGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCTC90400.11348413751616114No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG89350.1121660142374889No Hit
CTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG88540.11114917627965605No Hit
TGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCC87680.11006957054664833No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG86900.10909039325392039No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT85030.10674287846238033No Hit
CCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA84510.10609009360056171No Hit
GTTCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTC84390.10593945094014201No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT83820.10522389830314853No Hit
GCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCG83530.10485984520713428No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA82930.10410663190503586No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN82910.10408152479496594No Hit
CTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG80880.10153315312286627No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC80320.10083015404090775No Hit
TGCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG79840.10022758339922903No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG79690.10003928007370443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)