FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7965871
Sequences flagged as poor quality0
Sequence length35-101
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT2645233.320704038516315No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1539341.9324189407536225No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1126831.4145722420059275No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1024881.2865887484243719No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT713280.8954199735345952No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT689210.8652035665654139No Hit
GGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT572620.718841668412657No Hit
GGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT522180.6555215368162501No Hit
GCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT515590.6472487440482027No Hit
GTGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT494950.6213382064560171No Hit
CCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT454920.5710863256510179No Hit
AGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT372820.4680216388138849No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC332810.41779486511895564No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA319140.4006341553861467No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG318380.3996800852034887No Hit
GAGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT310790.3901519369319438No Hit
GTTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT308760.38760356525984413No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC307740.38632310264627684No Hit
TTTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT273990.34395485440324103No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC255180.32034161738245576No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT255110.32025374249721095No Hit
TGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT253240.31790622770567084No Hit
GTACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT225920.28360991535012303No Hit
GGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC224960.28240477406676556No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC219700.27580160411836946No Hit
CGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT210960.26482979701780257No Hit
GTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC210280.2639761552754244No Hit
CCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT209690.2632354955283609No Hit
TTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT206890.2597205001185683No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG199830.250857690263877No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC193970.24350130701338246No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC181630.2280102201002251No Hit
TGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT179370.22517311666232104No Hit
GACCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT179030.22474629579113192No Hit
GTCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT178590.2241939393695931No Hit
CTGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT173910.2183188756132255No Hit
GCACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT171570.21538134373504164No Hit
TTACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT168570.2116152772245496No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC160300.20123348721062645No Hit
TTGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT157910.19823318755726776No Hit
CGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT155170.19479351347768498No Hit
AGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT151140.18973443079859065No Hit
GAACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT150770.18926994926229662No Hit
TTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC150340.18873014639579275No Hit
AGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT149970.18826566485949872No Hit
GGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC148750.18673413114523194No Hit
ATTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT144170.18098460293921406No Hit
GTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC140850.17681682266760282No Hit
CAGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT135130.16963618918759796No Hit
TGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT129740.1628698230237472No Hit
GTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT128460.16126296797927056No Hit
ATGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT121900.1530278358763279No Hit
GTGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT120010.1506552139747179No Hit
GAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC118090.14824493140800296No Hit
GATCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT115430.1449056857687No Hit
TGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC108430.13611819724421848No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT105840.13286682649016032No Hit
GCTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT105220.13208850607799197No Hit
AGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT104450.131121882340299No Hit
GGTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT99920.12543512190945597No Hit
ACGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT97370.12223396537553771No Hit
TGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT94860.11908302306175936No Hit
TAACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT92730.11640911583930998No Hit
GGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC90390.11347158396112615No Hit
TATCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT87850.11028298098224287No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCG87780.11019510609699805No Hit
GGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG84020.10547496940384798No Hit
AGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC83910.10533688029846329No Hit
GGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA82470.10352916837342709No Hit
TAGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT82380.10341618637811233No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN81770.10265041952097895No Hit
CTTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT79830.10021502984419407No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)