FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9159156
Sequences flagged as poor quality0
Sequence length35-101
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG1832082.0002716407494314No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA1570921.7151361981387805No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA1548931.6911274357593646No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1114141.216422124483959No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA932951.0185982201853534No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG932271.017855793699769No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG893810.975865025118035No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG837640.9145384138014464No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG804380.8782250242271231No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG774230.8453071440206936No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA698510.7626357712435513No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG661490.722217199925408No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG540780.5904255806976101No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG507460.5540466829039706No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG472730.5161283419563986No Hit
CCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG462450.5049046003802097No Hit
TCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA452450.4939865638274967No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC448880.4900888247781783No Hit
TCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA414840.45292382835274336No Hit
CCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG384680.4199950301097612No Hit
CCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG340760.372043013570246No Hit
TCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG331060.36145251811411444No Hit
GTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG327520.3575875331744541No Hit
ACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA325100.35494536832869755No Hit
CCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG323730.35344959732097586No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC319490.3488203498226256No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG318400.34763028383837985No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG302540.33031427786577716No Hit
GCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA298010.3253684073073982No Hit
ATCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG288580.3150726988381899No Hit
CTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG279460.30511544950211567No Hit
TCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGG277460.3029318421915731No Hit
TCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG276500.3018837106825127No Hit
CCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGA275490.30078098899068867No Hit
TCCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA266660.29114036271464316No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG266010.2904306903387168No Hit
CCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA261870.28591062320589367No Hit
CCCCATACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG248430.27123678207904744No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG246540.2691732731705847No Hit
ACCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG229700.25078729961581614No Hit
ACCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA224730.2453610354491178No Hit
CCCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA222370.24278437882267753No Hit
CCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG220800.2410702470839016No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG209680.22892939043728483No Hit
TTCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA202070.22062076462067026No Hit
TCTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA194140.21196276163436895No Hit
GCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG190140.20759554701328375No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC188640.20595784153037683No Hit
GCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA188020.20528092326410863No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN187710.2049424641309745No Hit
CCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG183660.2005206593271258No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG180720.19731075658062816No Hit
CTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA176800.1930308862519647No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC173460.18938426204335856No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC173400.1893187538240423No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA173290.18919865542196246No Hit
CTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG169730.18531183440919666No Hit
GGTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCG167140.182484062942044No Hit
CCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGG165450.18063891476463553No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG162220.17711238895810924No Hit
GTTCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTC161150.17594415904696895No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG160170.1748741914648031No Hit
CCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA160010.1746995028799597No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG153860.1679849104000412No Hit
CCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA151970.16592140149157847No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG149540.16326831860926924No Hit
TTCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCG142530.15561477498581747No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT139430.15223018365447646No Hit
CCCCCTACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGG138550.1512693964378377No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC137940.15060339620812224No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG137080.14966444506458892No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG134590.1469458539629634No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG130520.14250221308600922No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA129170.141028278151393No Hit
TACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAAC124790.1362461781413047No Hit
TCCCCTCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGG124600.13603873544680317No Hit
CTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG121980.13317820986999238No Hit
CCCCTCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGG121890.13307994754101796No Hit
TGCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG120180.13121296329050405No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC117650.1284507000426677No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG113970.12443286259126933No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC113040.12341748519186702No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT111990.12227109135383217No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC109390.11943240185012681No Hit
CGTAGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG109320.11935597559425781No Hit
TGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC108230.1181659096100121No Hit
TTCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG108120.11804581120793226No Hit
CCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA106900.11671381074850128No Hit
TACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG105760.115469154581492No Hit
CGCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG101300.11059971027898204No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT101160.11044685776724407No Hit
CCCCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG101150.11043593973069134No Hit
CGGCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG98960.10804488972564721No Hit
CCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA98530.10757541415388056No Hit
AGAGGCGATGGGGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGC98160.10717144680143016No Hit
TCTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC97330.106265249767555No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA96180.10500967556399302No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG95150.10388511779906358No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA94540.10321911756934808No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)