FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12962594
Sequences flagged as poor quality0
Sequence length35-101
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG3290812.538697115716191No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA2948612.274706744653115No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA2926692.2577965490549188No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG1659111.2799212873596133No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1556621.20085532263064No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG1499771.1569983600504652No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG1346051.038410984714942No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG1100930.8493130310183286No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG958500.7394353321565112No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA936740.7226485686429738No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG907220.6998753490235057No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG759020.5855463806086961No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG727050.5608831071928967No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG699940.5399690833485952No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC659960.5091264911945865No Hit
TCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA637900.4921082925223146No Hit
CCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG632290.48778045505398065No Hit
TCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA611550.4717805710801403No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA604570.46639584638691917No Hit
ACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA569820.43958794050018074No Hit
GTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG540980.4173393072405106No Hit
GCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA536420.4138214928277473No Hit
CCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG522230.40287460981960865No Hit
CCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG479780.37012653485868646No Hit
TCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG476670.36772732371313954No Hit
ATCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG465500.35911022130292747No Hit
TCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG456180.3519203023715778No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC443860.3424160318528838No Hit
CCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG438310.33813448141629676No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG426920.3293476598896795No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG395160.3048463910849943No Hit
CTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG393770.30377407484952473No Hit
TCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGG386240.2979650523652905No Hit
CCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGGA380360.2934289232540956No Hit
TCCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA369810.2852901201719347No Hit
ACCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG362730.2798282504258021No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG340280.26250918604717544No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG338060.26079656587254063No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG336570.2596471045841596No Hit
ACCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA335140.2585439303275255No Hit
CCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA317460.2449046849727763No Hit
GCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG314390.2425363318483939No Hit
TTCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA313430.24179573934044377No Hit
GCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA303780.23435124173448615No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG298360.23016997986668408No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC293710.2265827349063004No Hit
CCCCATACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG290950.2244535314459436No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN290000.2237206534432846No Hit
CCCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA276050.21295891856213345No Hit
CCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG275540.2125654787922849No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG271840.2097111118345603No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC262320.20236690279738764No Hit
TCTCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA257560.1986947982788013No Hit
GGTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCG250680.19338721863849165No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC247630.1910342945246916No Hit
CCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG245930.1897228286251965No Hit
CTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA236030.18208546838696021No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG235180.18142973543721264No Hit
CTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG232440.17931596098743816No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC231090.1782745027731332No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG221870.171161728894695No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG217140.1675127678919821No Hit
CCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGG215690.1663941646247657No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT215680.16638645011947453No Hit
CTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG213850.16497469565119452No Hit
CCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA212170.1636786587622817No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG205340.15840965164842777No Hit
TGCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG204970.1581242149526553No Hit
GTTCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTC198470.15310978651340929No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC196450.15155145644459742No Hit
CCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA192050.14815707411649243No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG191420.1476710602831501No Hit
CCCCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG184320.1421937615264352No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG178760.13790449658455706No Hit
TACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAAC176800.13639245354749213No Hit
TTCCCCCCACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCG175880.13568271906070653No Hit
CCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA175130.13510413116387046No Hit
TACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG165130.1273896258727227No Hit
CGCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG162240.125160133843581No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC158380.12218233480119796No Hit
GCCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG155880.12025370847841103No Hit
CGTAGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG155100.1196519770657015No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC153630.11851794478790278No Hit
TGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC149450.11529328157620304No Hit
TTCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG147550.11382752557088498No Hit
CCCCCTACACGCGCGGGACACGCCCGCCCGCCCCCGCCACGCACCTCGGG139740.10780249693849858No Hit
CGGCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG138310.10669932268186444No Hit
TCTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC137960.10642931499667428No Hit
TCTGGGTGGTATTACACAGGGCCTGATGGGTTTTGGGAACTGCTCCTACG134530.1037832396818106No Hit
CCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG132240.10201661797013778No Hit
TGCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA131760.10164632171616268No Hit
TCGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG131390.10136088502039022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)