FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12962594
Sequences flagged as poor quality0
Sequence length35-101
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT4303873.320222788741204No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT2613682.0163248189367033No Hit
GGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT2028111.5645865326029649No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1707851.3175217861486674No Hit
GGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1586091.2235899697236525No Hit
TTTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1470761.1346185802008457No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1330331.0262837823972577No Hit
GTTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1239480.9561975018271807No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1192710.9201167605804826No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT1158960.894080305222859No Hit
GTGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT987290.7616453928897257No Hit
AGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT922770.7118714047512403No Hit
TGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT855760.6601765047952594No Hit
TTACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT852880.6579547272714088No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC823020.6349192144720416No Hit
GTACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT817800.6308922427100625No Hit
ATTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT814040.627991588720591No Hit
GCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT799390.6166898384690594No Hit
CCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT763100.5886938987674843No Hit
CGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT758940.5854846645663668No Hit
AGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT754270.5818819905954009No Hit
GGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC733870.5661443998014595No Hit
TTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC657600.5073058679458756No Hit
GAGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT629220.4854121019295983No Hit
TGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT607270.4684787628155291No Hit
TGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT568790.4387933464551926No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC557100.4297750897698408No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA534210.4121165871584036No Hit
GAACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT531470.41000281270862915No Hit
CGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT503680.38856420250452955No Hit
GCACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT500500.3861109898219446No Hit
TATCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT485340.3744157998005646No Hit
GATCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT472380.3644178009432371No Hit
TAACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT471830.363993503152224No Hit
GTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC460710.3554149732684677No Hit
AGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT457870.35322405376578175No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC410100.316371861989969No Hit
TTGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT408660.3152609732280437No Hit
ATACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT378600.2920711703228536No Hit
GGTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC374820.28915508732279976No Hit
AGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT371830.28684845024074657No Hit
CTGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT368360.28417151690471826No Hit
GCTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT358930.276896738415166No Hit
TGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC358310.27641843908711483No Hit
ATGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT342310.26407523062127847No Hit
CCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT339690.26205403023499774No Hit
GACCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT339170.26165287595985803No Hit
GTCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT336500.2595931030471216No Hit
CTTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT327590.25271947883270895No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC323690.24971082176916132No Hit
GTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC308990.23837049899117416No Hit
AATCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT308080.2376684790096797No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC305530.235701280160437No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG295780.22817963750156794No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC289640.22344293125280326No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN287780.2220080332686498No Hit
GGTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA283800.21893766016277297No Hit
CAGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT275100.21222604055947444No Hit
TTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT266100.20528298579744145No Hit
TTTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC246580.19022427146912108No Hit
TTTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA241050.18595815004311636No Hit
ACGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT239490.18475468721769733No Hit
TTTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG236980.18281834638961925No Hit
TAGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT227050.17515784263550951No Hit
GTTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC217120.1674973388813998No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG213460.16467382994483976No Hit
TGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT211370.16306149833898986No Hit
TGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC211010.16278377614850856No Hit
AAGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT208970.16121001706911442No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC208220.16063142917227832No Hit
GGAGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC203830.15724476134946447No Hit
GGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC202640.1563267352198179No Hit
TATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC195050.15047142570383676No Hit
AAACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT194780.15026313406097574No Hit
AGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC193680.1494145384789495No Hit
GTGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC187010.14426896344975396No Hit
CCTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT183090.14124487737562405No Hit
ATTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC182500.14078972156344632No Hit
GGGGAAAATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCG180770.13945511214807776No Hit
TTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG180740.13943196863220433No Hit
GGTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC176140.13588329619827635No Hit
GTTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA174370.1345178287617432No Hit
GAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC172340.13295178418764023No Hit
GGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG169870.13104630138072673No Hit
CCACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT165060.12733562433568466No Hit
AGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC164890.12720447774573515No Hit
ATTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC163380.12603958744677185No Hit
CTACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT163250.1259392988779869No Hit
CGTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC162700.12551500108697378No Hit
TCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT162650.12547642856051805No Hit
GGTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC159800.12327779455254095No Hit
CATCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT159720.12321607851021175No Hit
GATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC157280.12133373921917172No Hit
AGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC156790.12095572845990549No Hit
AGTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC156180.12048514363714546No Hit
TCACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT153330.11828650962916835No Hit
TTTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC151450.11683618263443257No Hit
GGGCGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC147200.11355751788569479No Hit
TGTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC146560.11306378954706134No Hit
TTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC145130.11196061529042721No Hit
CGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC142360.10982369732477927No Hit
TTTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC138010.10646788752313001No Hit
ACTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT131710.10160774918970693No Hit
GGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTCC130510.1006820085547692No Hit
TGTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA130270.10049686042778165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)