FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12745627
Sequences flagged as poor quality0
Sequence length35-101
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA7542515.917723780870097No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG2105031.6515703778244886No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1951281.5309407689398096No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC1534301.2037854238163412No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1428161.1205098030877572No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT1042820.8181786584528167No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA989690.776493773119204No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG938840.7365977366197834No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG903650.7089882671131048No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG799350.6271562787770268No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG711960.5585915859612085No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC699840.5490824421583967No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA697380.5471523684162419No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT592020.464488722288829No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC565940.4440268022906994No Hit
TCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC542800.4258715557892915No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC534510.41936736419479403No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG495630.38886278407488306No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG477960.37499920560989275No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG435710.341850581379794No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC395540.3103338894194848No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC388120.30451228488013965No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG387860.304308293346416No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG374890.2941322541448922No Hit
GGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA371950.29182558064817055No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA368850.28939337389992664No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC364720.2861530468450081No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC360770.2830539446980521No Hit
GGCTCCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA338380.2654871353131548No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN329690.2586691105898517No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC322560.2530750350688907No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG317830.24936395832076363No Hit
GGCCGCGACGCCCGCCGCAGCTGAGGCGATCCACGGGAAGGGCCCGGCTC317380.24901089605085727No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC301100.23623788770846657No Hit
CCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTG300990.23615158359804506No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC298060.2338527559295435No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG297730.23359384359827887No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG296490.23262096089898127No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG295360.23173438231010526No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG293940.23062027470284516No Hit
GGCCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA286290.22461821611443675No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC260320.20424260022672874No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG254340.19955079495108402No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC244700.1919874165468674No Hit
TGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG242120.18996319286607086No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG238290.18695824065775657No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG236740.18574213728363462No Hit
GAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCG233020.1828234891857419No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC229570.18011667844979304No Hit
CACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCC225250.1767272806586918No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG222620.17466382783679452No Hit
GGCTACCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA215390.16899129403363208No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG215360.16896775654897167No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG212340.16659831642648887No Hit
GGGTCAGTGAAAAAACGATGAGAGTAGTGGTATTTCACCGGCGGCCCGCG210850.16542928802168777No Hit
GTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG208160.16331876023046962No Hit
GGCCTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA207680.16294216047590282No Hit
CCCCGGGAGCGGCCGCGACGCCCGCCGCAGCTGAGGCGATCCACGGGAAG205590.1613023823778932No Hit
TTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGC199920.15685379777707287No Hit
GCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGAC193610.15190308017016346No Hit
GGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCG189540.1487098280845658No Hit
TCCCTACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC189430.14862352397414422No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC187840.14737603728714171No Hit
CGTAGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG184680.1448967555695769No Hit
TACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAAC183200.14373557299299594No Hit
GCGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA181070.14206441158210575No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG179510.14084046237976366No Hit
CGGCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG179490.1408247707233234No Hit
CGCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG177450.13922422176641447No Hit
GTGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA173600.13620357790165993No Hit
TGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC170490.13376352532519584No Hit
TCCCCGCCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC170410.1337007586994347No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA170230.13355953379147215No Hit
ATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCT169330.13285340925165942No Hit
TCCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCT160420.12586277630751316No Hit
TCTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC159480.12512526845481983No Hit
AGTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG159390.12505465600083857No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC157070.12323442385376569No Hit
GGCACTGTCCCCGGAGCGGGTCGCGCCCGCCCGCACGCGCGGGACGGACG153670.1205668422589175No Hit
GGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC153410.12036285072519384No Hit
TCGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG152270.11946842630809767No Hit
GGCTGCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA151510.1188721433633669No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA150240.1178757231794089No Hit
GCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG149650.11741281931442055No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG148780.11673023225926822No Hit
GGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGA148120.11621240759673887No Hit
GGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA146150.11466677943737094No Hit
AGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG145410.11408618814908046No Hit
CCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG143300.11243071839463056No Hit
GGGTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA142800.11203842698362348No Hit
CAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCC142690.11195212287320193No Hit
TAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGC141470.11099493183034463No Hit
GTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGG139680.10959052857893926No Hit
TGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG139440.10940222870165588No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAC134980.10590298931547268No Hit
ATGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGA134690.10567546029708857No Hit
GACGGGTCGGGTGGGTAGCCGACATCGCCGCCGACCCCGTGCGCTCGGCT134520.10554208121734615No Hit
AGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG134500.10552638956090588No Hit
GGCATCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA134480.10551069790446557No Hit
GGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAG134360.10541654796582388No Hit
CCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTG132060.1036120074751913No Hit
AGCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG131170.1029137287635987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)