FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13732659
Sequences flagged as poor quality0
Sequence length35-101
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG162279311.817034122816274No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA3770592.745710062413987No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC2580921.879402961946408No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG2155151.5693610392568547No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC1605351.1690015750045202No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1569041.1425609563304528No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG1448351.0546755730263164No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1184280.8623821504633589No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1029420.7496144774293165No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG980950.7143190550351538No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA771280.5616392280620963No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG755920.5504542128367128No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT692910.5045708919153967No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG616480.44891524649377806No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT598880.4360990832146928No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA592550.4314896335807945No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC570910.4157315782762828No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG568900.4142679141745237No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG561970.4092215498833839No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG554450.4037455528459565No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA524340.381819718963385No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG510430.37169058082633527No Hit
CGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCC496390.36146677784688314No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA494560.3601341881422964No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG484330.35268479323632806No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC416810.3035173304747464No Hit
AGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG403610.2939052080154324No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA400210.29142935829106364No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG399490.2909050607023738No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG396600.2888005884366604No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC396310.28858941301899366No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG386070.28113273620207124No Hit
GGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG384780.28019336968900194No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC375000.2730716607759648No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA374670.2728313577144819No Hit
TGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG361230.26304446939227133No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG355250.25868988664176396No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC341720.24883746112096716No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG338500.24649268579377087No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC334210.24336874599449385No Hit
GCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCCC334060.24325951733018347No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN327870.23875201444964156No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG317310.2310623164821904No Hit
CGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG314410.2289505623055229No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG312520.22757428113521203No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG291720.21242790635083852No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG289950.21113900811197597No Hit
GAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGG279040.2031944432611339No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG272990.19878888713394832No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC268980.195868840841384No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG256840.1870286009431968No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG253090.18429788433543715No Hit
TAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG251860.18340220928809198No Hit
GGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGG250750.18259391717219514No Hit
GAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG247730.18039478006407936No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG241060.1755377454577442No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC227800.16588193153270608No Hit
GTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG225430.16415611863660198No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG218470.15908790861260008No Hit
TGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG216460.15762424451084092No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG215340.15680867048399003No Hit
TGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG213620.1555561817998976No Hit
GGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA209160.15230844951440214No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC207910.1513982106451489No Hit
AAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG206580.15042971648826348No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG206540.1504005888444474No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC205880.1499199827214817No Hit
TGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA204440.14887138754410198No Hit
TCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC204240.14872574932502147No Hit
AGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA202190.14723295757944618No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG200280.1458421125872273No Hit
GGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGG196600.14316236935614582No Hit
GGCTCCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA195790.14257253456886973No Hit
TGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG193200.14068651963177706No Hit
CGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGC190690.13885875998231662No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC188820.1374970426339138No Hit
ATGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG183290.13347014587633757No Hit
CAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG182140.1326327261166246No Hit
GTGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG181580.13222493910319916No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG174870.1273387768530479No Hit
GGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAA174590.1271348833463352No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG173850.12659602193573727No Hit
GGCCGCGACGCCCGCCGCAGCTGAGGCGATCCACGGGAAGGGCCCGGCTC173290.12618823492231185No Hit
CCCGCTCCGGAACCCCCGCGGGGTTGGACCCGCCGCCCCGGAGCCCGCGC171900.12517604929970227No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG171110.12460077833433424No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA167290.1218190883498964No Hit
TTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC167030.12162975866509174No Hit
CAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCC165150.1202607594057349No Hit
GCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG157300.1145444593068247No Hit
AGTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG156470.11394006069764057No Hit
TGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCC154310.11236716793157102No Hit
GAGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCG152010.11069232841214509No Hit
GGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC151530.11034279668635186No Hit
TAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG150550.10962916941285733No Hit
TGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG150040.10925779195420202No Hit
GATGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG149010.10850775512593737No Hit
GTTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG143350.10438619352595882No Hit
GTGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG142900.10405850753302764No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG142570.1038182044715448No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG142290.10361431096483208No Hit
GCCGGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC141740.10321380586236067No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG140110.10202685437685446No Hit
TAGGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG140100.10201957246590045No Hit
AGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG137960.10046124352173895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)