FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11890194
Sequences flagged as poor quality0
Sequence length35-101
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC2186961.8392971552861122No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC1154390.9708756644340707No Hit
GGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC918270.7722918566341306No Hit
TTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC874980.7358837038319139No Hit
GTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC771480.6488371846582149No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG724740.6095274812168751No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC673740.5666349935080959No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC671400.5646669852485166No Hit
GTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC627450.5277037531935981No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC539710.45391185375108256No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA532330.447705058470871No Hit
TTTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC519440.4368641924597698No Hit
TTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT475760.40012803828095656No Hit
GTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC461360.38801721822200713No Hit
GGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC426150.35840458111953427No Hit
GTTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC416460.35025500845486623No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG416070.3499270070782697No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC388720.32692485925797343No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC363950.3060925666982389No Hit
TGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC355180.2987167408706704No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC351050.29524329039543No Hit
GAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC334790.2815681560788663No Hit
GGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC333220.28024774028077254No Hit
GGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG321050.27001241527261877No Hit
AGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC280380.23580775889779423No Hit
TTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG271000.22791890527606193No Hit
TGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC264530.22247744654124232No Hit
GTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG252610.2124523788257786No Hit
GATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC252040.21197299219844523No Hit
ATTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC251610.2116113496550182No Hit
GTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT251490.21151042615452698No Hit
TATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC250480.21066098669205902No Hit
CGGGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC248660.20913031360127515No Hit
GGAAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC245240.20625399383727466No Hit
TTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC241720.20329357115619812No Hit
ATTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC238610.20067797043513336No Hit
TTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC238470.2005602263512269No Hit
TTAAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC232470.19551405132666463No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT227480.19131731576457037No Hit
GGTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA217850.18321820485014795No Hit
GTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG216140.1817800449681477No Hit
AGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC215560.18129224804910668No Hit
TGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC207410.17443786030740963No Hit
GTAAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC205320.17268010934052044No Hit
TGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC202370.17019907328677733No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC198400.1668601874788586No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC197370.16599392743297545No Hit
GGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC196780.1654977202222268No Hit
TTTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA189650.15950118223470533No Hit
ATTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT185590.1560866038014182No Hit
ATGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC184180.15490075267064607No Hit
CGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC182180.15321869432912533No Hit
GGTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC173310.14575876558448078No Hit
GTTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA170070.14303383107121717No Hit
CTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC167420.14080510376870217No Hit
AGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC163420.13744098708566066No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT162050.13628877712171897No Hit
AATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC159330.13400117777725074No Hit
GGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG156770.13184814310010418No Hit
GCTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC156080.1312678329722795No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC155780.1310155242210514No Hit
GGTGTGGTGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGAC155260.130578189052256No Hit
TTTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC155060.13040998321810393No Hit
AGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC150990.1269869944931092No Hit
GGTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT146740.12341262051737761No Hit
CAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC143520.1207045065875292No Hit
GTGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA139960.11771044273962224No Hit
CGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC137570.11570038302150494No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT137160.1153555610614932No Hit
GTTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC136260.11459863480780885No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN135760.11417812022242867No Hit
AGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG128310.10791245290026387No Hit
CGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCG126590.10646588272655601No Hit
TGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG124610.10480064496845047No Hit
GTCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC124260.10450628475868434No Hit
GGGCGGCGGGGGACCGCCCCCCGGCCGGCGACCGGCCGCCGCCGGGCGCA122790.10326997187766658No Hit
CTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC121980.10258873824935068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)