FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13911285
Sequences flagged as poor quality0
Sequence length35-101
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2601471.8700429183932326No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG2544601.8291624389838896No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1827401.3136097779608427No Hit
GTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1791501.2878033912755005No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1725211.2401514310144606No Hit
TTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1709131.2285924700701625No Hit
GGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1599371.1496924978533616No Hit
GTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1166020.8381828134496563No Hit
GTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG1139820.8193491830553395No Hit
GGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC1036100.7447910096011979No Hit
ATTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG912430.6558919610948952No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT886220.6370511422920312No Hit
GGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC869980.625377166810974No Hit
TGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG862910.6202949619679275No Hit
AGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG846750.6086784937552497No Hit
TTTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC812230.5838641074494556No Hit
GTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC730610.5251923168851763No Hit
TTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG623520.4482116497505443No Hit
AGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG616650.44327321307844675No Hit
GGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA596010.4284363378365119No Hit
CGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC577170.41489337613311783No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT566170.4069861267309238No Hit
TTTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT547280.3934072229847926No Hit
TGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC540310.38839690222722056No Hit
CGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC535890.3852196256492481No Hit
TATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG530230.3811509864113919No Hit
TGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG527690.37932513064033985No Hit
TTTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA525380.3776646082658791No Hit
AATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG516420.37122379420736473No Hit
GTTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC515460.37053370698680965No Hit
TATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC491460.3532815264729319No Hit
GTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC468750.336956650661675No Hit
ATTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC464450.3338656349862719No Hit
GGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT464020.33355653341873165No Hit
GTTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT453910.3262890523772606No Hit
CGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG449060.3228026742317478No Hit
GCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC426730.30675095794529406No Hit
ATGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG416940.2997135059773414No Hit
TTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT410870.29535014198903986No Hit
GGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC408340.2935314746265352No Hit
GAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG393760.2830507749643545No Hit
TTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC386230.2776379033281253No Hit
GATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG384540.2764230622836064No Hit
ATTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC384080.2760923954904238No Hit
GAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC378900.2723687998628451No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG378330.27195906057564057No Hit
TTTGGCAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGG376980.2709886254217349No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG375490.269917552548165No Hit
GTTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA375320.2697953496028584No Hit
GATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC370900.2666180730248859No Hit
TTAAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC352420.25333389402920004No Hit
GGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT350310.25181713982568826No Hit
GGTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA347500.24979719702385508No Hit
GGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT336020.24154490401138354No Hit
GTGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT331950.23861922173257177No Hit
TTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC330570.23762722135302383No Hit
TGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC323640.23264565422964162No Hit
CTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG322690.23196275541763398No Hit
TTACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT314730.2262407822138645No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC312650.22474559323599508No Hit
AAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG308710.22191336026830014No Hit
GGGGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC301190.21650767704061846No Hit
AATGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC301140.2164717349978812No Hit
TTTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC299200.2150771837396761No Hit
AGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC299040.21496216920291691No Hit
ATTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT296290.2129853568523684No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC295130.21215150146086434No Hit
GTACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT289630.20819787675976734No Hit
CCGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC288870.20765155771016122No Hit
TTTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC287080.2063648325801678No Hit
AGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC267750.1924696388579488No Hit
CGCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT263620.18950082612785232No Hit
GGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC262530.18871728959618037No Hit
AGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC257650.18520934622502522No Hit
CTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG252320.18137792446923487No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN252290.18135635924359253No Hit
TGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC249200.17913514100243075No Hit
ATGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC248290.1784809958246129No Hit
GTAAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC247880.17818627107416748No Hit
GCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT246670.17731647363992614No Hit
GTGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA238330.17132134091135365No Hit
CAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG236170.16976864466510463No Hit
AGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT231040.16608099108026325No Hit
GGAAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC227310.16339971469206477No Hit
TGGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC223220.16045965559615807No Hit
TGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT211540.15206359441273756No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC209830.15083437655112378No Hit
GAGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT205560.14776492610136305No Hit
TAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG204630.14709640410645028No Hit
GTTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC203720.1464422589286324No Hit
CGTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC203080.14598220078159566No Hit
GTTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC200260.143955069571215No Hit
AGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT191680.13778741503750372No Hit
GGTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGGAC190760.13712608145113842No Hit
GGTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAACGGAAC190730.13710451622549605No Hit
AGTAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC189950.13654382035879503No Hit
ACGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG189880.1364935014989629No Hit
TAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC180940.13006706425754344No Hit
TATCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT178690.12844967233436738No Hit
CCGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT173510.12472607670678877No Hit
TGTGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA172660.1241150619802556No Hit
GAACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT172500.12400004744349642No Hit
CGTCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT169440.121800394427977No Hit
AAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC169200.12162787262283822No Hit
ATGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC166660.11980201685178615No Hit
TAACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT163680.11765987110464633No Hit
GCTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC163490.11752329134224479No Hit
GATCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT162230.11661755186526622No Hit
CTGAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC160170.1151367397044917No Hit
TTTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC158100.11364873913516976No Hit
CAGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC157210.1130089707744468No Hit
GCCCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT155110.11149940497948248No Hit
TTGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT153500.11034207120334319No Hit
CGCAGCCGGGTCCCCGTCCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCC153250.11016236098965695No Hit
GTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT152940.10993952032468603No Hit
CATGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG152810.10984607101356919No Hit
ATTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT151840.10914879538446663No Hit
CTTGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC148380.10666160602704926No Hit
GGTGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC148070.10643876536207833No Hit
GGGGGTGGCGCGCGGGCGGGGCCGGGGGTGGGGTCGGCGGGGGACCGCCC146240.10512328659789516No Hit
TGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT145600.10466322845085843No Hit
GCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG143240.10296676403366044No Hit
CGGGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTCGGAA139680.10040769059076857No Hit
CTGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC139620.10036456013948387No Hit
TGACGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT139310.10014171947451296No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)