FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10770377
Sequences flagged as poor quality0
Sequence length35-101
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG8666448.046552130904981No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA2006001.8625160474884026No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC1552331.441295880357763No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1536241.4263567561284065No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC1495121.3881779625727122No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG1332551.2372361710272537No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG940030.8727921037490146No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG926980.8606755362416747No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG815750.7574015282844788No Hit
CCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG747870.6943768077941933No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT723710.6719449096350109No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG716770.6655013097498816No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG641130.5952716418376071No Hit
TCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA638600.5929226061446131No Hit
CCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA604570.561326683364937No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG590940.5486716017461599No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC561820.5216344794615825No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC555570.5158315256745423No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG441950.41033846818918224No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA420730.39063627949142354No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC414230.38460120755290184No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG413760.38416482542811636No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC387300.35959744027530327No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC387020.3593374679456439No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC375630.3487621649641419No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG375590.34872502605990485No Hit
CCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG368480.342123585831768No Hit
GTTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGG368320.34197503021481973No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG360020.3342687075856305No Hit
AGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG353260.32799223276956785No Hit
TTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG328360.30487326488199995No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT327910.30445545220933307No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG322750.2996645335627527No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA313250.2908440438064517No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG309630.2874829729729981No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC301390.2798323587001644No Hit
TGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCG293870.27285024470359764No Hit
CCCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG288260.26764151338435044No Hit
TCCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA279590.25959165589096833No Hit
CGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCC258860.24034441877011362No Hit
CCCCTCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG252200.23416079121464364No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG251220.23325088806083577No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG248320.23055831750364914No Hit
GGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGG240450.22325123809500816No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA240110.2229355574089932No Hit
GAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG238110.22107861219714037No Hit
GTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG235700.21884099321685768No Hit
CCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAGG233340.21664979786687133No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC233040.21637125608509342No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA231420.21486713046349257No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG229570.21314945614252873No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG222150.20626018940655466No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG220520.20474677905889463No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG219680.2039668620699164No Hit
AGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG216320.20084719411400362No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN213230.19797821376169097No Hit
CGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGC206140.19139534298567265No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC203370.18882347386725645No Hit
TGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG197320.1832062146014016No Hit
CAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCC192280.17852671266753245No Hit
CGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG184440.1712474874370693No Hit
GCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTCTCCC182830.16975264654152775No Hit
TCCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA182830.16975264654152775No Hit
GCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG180710.16778428461696374No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG180400.16749645810912656No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG176130.16353188008182074No Hit
TCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA170550.1583510029407513No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC170440.1582488709540994No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAG166340.15444213326980105No Hit
CCCTCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG164120.15238092408464438No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC162240.15063539558550273No Hit
TAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG161030.14951194373233173No Hit
GGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGG160190.14873202674335353No Hit
GGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC158140.1468286579012044No Hit
TGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC154020.14300335076478754No Hit
TACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAAC152360.14146208623894968No Hit
TGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGG149480.13878808513388158No Hit
GCCGGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC148630.13799888341884411No Hit
TCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG148010.13742323040316973No Hit
CGTAGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG147080.13655975087965816No Hit
ATCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG144100.13379290251399742No Hit
TGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC142630.1324280477832856No Hit
ACCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA141090.13099819997015888No Hit
AAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG138380.12848203920809828No Hit
GCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGA137050.12724717064221616No Hit
ACGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG134170.12457316953714806No Hit
TCTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC134160.12456388481108878No Hit
CGGCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG133280.12374682891787353No Hit
CGCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG133000.12348685658821414No Hit
GTCCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGG132780.12328259261491033No Hit
GAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGG132140.1226883701471174No Hit
TCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG130460.12112853616916101No Hit
GTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTT126760.11769318752723326No Hit
CCCCGCACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGAG126710.11764676389693694No Hit
TCGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG123500.11466636683191311No Hit
GGAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCG122880.11409071381623874No Hit
CCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCC120530.11190880319231165No Hit
CCTCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG120010.1114259974372299No Hit
CCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG118190.10973617729444383No Hit
CAGGGGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGG117220.1088355588666952No Hit
GGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA116240.1079256557128873No Hit
GGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGG115800.10751712776627968No Hit
AGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG114640.10644009954340504No Hit
TCCCCCACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAG114320.10614298830950858No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113240.10514023789510803No Hit
TGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGG111890.10388679987710736No Hit
AGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG111620.10363611227350723No Hit
CCCCTACACGCGCGGGACGCCCGCCCGCCCCCGCCACGCACCTCGGGAGA110520.10261479240698816No Hit
TGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGA109470.10163989617076542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)