FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361432
Sequences flagged as poor quality0
Sequence length35-101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA235360.9966833683968033No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT220730.9347294353595615No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA172190.7291761947835043No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG122700.519599971542691No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC114390.4844094600225625No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT113640.4812334210767026No Hit
TGGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCT105550.4469745476473597No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG79740.337676460723832No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA79200.3353897126828128No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA77620.3286988573035345No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG71390.3023165604599243No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT68110.28842668347003003No Hit
GGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTC66550.28182052246264133No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT65570.27767049824005097No Hit
TTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG54660.2314697183742746No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT53910.22829367942841464No Hit
GGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCATAG53890.22820898505652504No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG53130.22499059892472023No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT49850.21110072193482599No Hit
TCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCA49470.20949152886892358No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA47620.2016572994691357No Hit
TGGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTAC44620.1889531436856958No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA43650.18484546664905024No Hit
GTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTA43280.18327862076909265No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA42800.18124595584374228No Hit
GGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACT41550.17595255760064232No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT41490.17569847448497353No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA37600.15922541915244648No Hit
CTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTA37420.1584631698054401No Hit
GGGTTAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA35910.15206874472777535No Hit
GGTCCTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTAT33990.1439380850263738No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG33970.14385339065448424No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC33140.14033857422106585No Hit
CTCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGC31590.1337747603996219No Hit
GAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATAC30000.1270415578343988No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT27120.11484556828229651No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA26740.11323637521639411No Hit
TTCGTTATTGAGTTTAGGTCCTAACAATGAATTTTCACATATAAGTTGGA25800.10925573973758296No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG25640.10857818476246617No Hit
GTTGGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGC24590.10413173023826221No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC24520.1038352999366486No Hit
ATGGCTAGTAGTTCTCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGG24470.1036235640069246No Hit
TGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTA24380.10324243933342142No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG24240.10264957873019423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)