FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361432
Sequences flagged as poor quality0
Sequence length35-101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT270341.1448138248317121No Hit
GTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT149080.6313118480650723No Hit
ATTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT101730.4307979226164463No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT95270.40344164049610576No Hit
GGTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT84300.35698677751466057No Hit
TATGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT71050.3008767561378011No Hit
GTGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT61700.2612821372794135No Hit
AATGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT57590.24387744385610086No Hit
TTAGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT55870.23659372787359534No Hit
TGTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT49870.21118541630671558No Hit
GATGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT42410.17959441559189507No Hit
TAAGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT42340.1792979852902815No Hit
CGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT40140.16998160438242557No Hit
TTGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT39790.1684994528743576No Hit
GTAGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT33290.14097378201023786No Hit
AGTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT32260.1366120218579235No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG32070.1358074253249723No Hit
TTTCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA31400.13297016386667074No Hit
TTTACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT30640.12975177773486596No Hit
ATAGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT28860.12221397863669163No Hit
GAAGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT28140.11916498124866605No Hit
TTAACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT27970.11844507908760446No Hit
CTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT27700.11730170506709488No Hit
GAGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT25840.10942512848136215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)