FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4766734
Sequences flagged as poor quality0
Sequence length35-101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA989222.0752573984619236No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT419810.880707838952205No Hit
GGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA364120.7638773214532215No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC229370.48118900698046085No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTTAATTATT224730.4714548787492652No Hit
TGGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCT188590.3956377679140476No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG186760.3917986613056235No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA177280.37191083035050837No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG147160.30872291174628164No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC142710.29938737928317377No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA135620.2845134635161098No Hit
GGGCCATTAGGTTTAATAAATTAAATAGATAAGTTATATAATTTAAGCTC129810.2723248245024791No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT127750.2680032072274224No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA122130.25621316398187943No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT106240.22287797053496167No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA102720.21549345946302018No Hit
GGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCATAG102090.21417179981093973No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG101030.2119480549995028No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG97720.20500409714492143No Hit
GCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCTAT96990.20347265024647904No Hit
TTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG93830.19684337326144066No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA92320.19367558584137484No Hit
TCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCA90830.19054975587058143No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG90230.18929103239240958No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG88490.1856407343057112No Hit
TGGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTAC81010.1699486482778355No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA79930.1676829460171262No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT79660.16711652045194886No Hit
GTGTGCGTACTTCATTGCTCAATTCAATTAAGCTCTCTATTCTTAATTTA79220.16619345656795617No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG78780.16527039268396349No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG78360.1643892862492432No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT77040.16162009459726512No Hit
CTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTA74260.15578800914840224No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT73200.15356426433696532No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA72240.15155030677189035No Hit
AGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAA71980.1510048599313492No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN71910.15085800885889586No Hit
GGTCCGTTGATCAAAATATATCTGGGTCAATAAGATATGTTGATTTTACT71720.1504594130908081No Hit
CGGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCA71000.14894894491700186No Hit
GGGTTAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTTATA70820.14857132787355032No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC67090.14074626358424866No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG64080.13443166746875324No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG59170.1241311136723803No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT58470.12266260294784648No Hit
GAGTTGTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATAC58130.12194932631021575No Hit
CTCTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGC56410.11834098567278979No Hit
GGTCCTAACAATGAATTTTCACATATAAGTTGGATTTTAATTCTATTTAT56240.11798434735397444No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG55950.11737596433952471No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC52720.11059983628203293No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG52010.10911034683286293No Hit
TCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGTCTAGGTT50530.10600549558670569No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT50060.10501949552880442No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC47910.10050906973202198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)