FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11765198
Sequences flagged as poor quality0
Sequence length35-101
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA5678574.826582604049673No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG1422811.2093379133950828No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1207971.0267315518191873No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC1185871.0079473375628696No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT912360.775473561940904No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG844050.7174124906355167No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA840010.7139786342737283No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG762640.6482168850876968No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA743440.631897567724742No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG638710.5428807912965No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG635770.5403818958252976No Hit
TGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG572420.4865366481720069No Hit
GGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA548870.46651998546900786No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT502100.4267671483301853No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC491840.4180465131143564No Hit
TGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG450360.3827899878948064No Hit
CGTCTCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG405060.3442865984915851No Hit
CGTCACGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG393120.33413802300649764No Hit
CGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC388330.3300666933102188No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC376520.3200286132031097No Hit
GGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA341170.28998237003746136No Hit
GGCTCCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA334000.2838881249597329No Hit
CGTTGTGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG324320.2756604691225766No Hit
GGGTTTTATAGAGGGGGTAAGAGCAGGGTAAAGGGCAGATCGGGGCGGAG304970.2592136570927238No Hit
CTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG286530.2435403127087194No Hit
CGCCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG263460.22393163293979412No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC258950.22009829328839176No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC255980.21757389888380968No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC241510.20527491335037454No Hit
GGCTACCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA227080.1930099263947789No Hit
GTTTCAGTATAATGGAAATAGGCAGGCGTATATGGGGTTGAATCCTCTAT224250.19060452701263506No Hit
GGCCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA221060.18789314042993582No Hit
CGTTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG213230.18123791881785584No Hit
GGCCTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA211800.1800224696600941No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC208680.17737058058861396No Hit
TGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG207070.17600213783057456No Hit
GGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG205640.17478668867281283No Hit
GGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGA205280.17448070147225742No Hit
CTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC200300.17024787853124104No Hit
AGTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG195780.16640603923537878No Hit
CGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG192930.1639836405643152No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN190800.1621732162943624No Hit
GTGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG185990.15808488730916387No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG185280.1574814125525129No Hit
CCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTG183330.15582398188283786No Hit
GGCTGCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA175560.14921975813751712No Hit
TCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCC173400.1473838349341847No Hit
AGCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG172150.14632137937670067No Hit
TGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAG171850.1460663900429045No Hit
CGACGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG171510.14577740213126883No Hit
ATGGTCTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGA171350.1456414078199109No Hit
CGTGGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG168480.14320200985992756No Hit
GTGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA163630.13907968229688952No Hit
ATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCT162260.13791523100588704No Hit
GGGTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA159450.13552683091266293No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC158000.13429438246598144No Hit
ACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCAC154510.13132800654948604No Hit
TAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGC152600.12970457445765043No Hit
GCGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA141730.12046546092976931No Hit
GCGCTAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG141220.12003197906231582No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG140330.11927551070538718No Hit
AGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCG139240.11834904945926111No Hit
CGTAGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG138670.1178645697250484No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCC136330.1158756529214383No Hit
TACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAAC134940.11469420234151606No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG133760.11369124429525114No Hit
GGCATCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA133650.11359774820619253No Hit
GCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGAC129800.11032538508914172No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC127960.10876145050852523No Hit
TTTGGGTCCTAACGCGTACGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC127530.10839596579675073No Hit
TGTCCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGC123380.10486861334590375No Hit
CGGCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG122470.10409514570005537No Hit
CGCTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCG119770.10180024169588987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)