FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11485935
Sequences flagged as poor quality0
Sequence length35-101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2203181.9181546822265667No Hit
GGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG1964821.7106313068983934No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1556161.354839636477135No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA1063140.9256016162375985No Hit
GCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCC716730.6240066655435539No Hit
GGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCT677970.5902610453567776No Hit
GGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCC552700.4811972207747998No Hit
GCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGG495950.4317889662443676No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG404410.35209149276919993No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGC388060.3378566916842208No Hit
GCGTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCG386930.336872879743791No Hit
CTGGCAAATAGTTTTGTTAAATTTAATTATTTAGGTTTATGGCTAAGCAT369680.32185451162661116No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGG334970.291634943084738No Hit
CGTTGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG306560.2669003437682696No Hit
CCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCC264770.2305167145730844No Hit
TGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC258700.22523199025590865No Hit
TGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG258540.22509268945018404No Hit
GAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAG254260.22136639289705193No Hit
CCGCTGACTAGTTTCTTTTCCTCCGCTGACTAGTTTCTTTTCCTCCGCTG239750.20873355107790528No Hit
CGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC224030.1950472469154666No Hit
AGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC223010.19415920427897249No Hit
TTTTGGATCTTTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTA219700.19127741886054553No Hit
TTCCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATT211600.18422531557073935No Hit
AGAGGCGATGGGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGCC205180.17863587074104112No Hit
AGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGC204820.17832244392816085No Hit
GTTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG202520.1763199948458702No Hit
GGTTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA183330.15961260445927997No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG183160.15946459735319762No Hit
GTTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGC169650.14770238556982954No Hit
AGAGGCGATGGGGGGGGTGGAGCGAGGCCCCGCGGGGAGGGGACCCGCGC165120.14375843150775275No Hit
GCTCTGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG158910.138351818985568No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGT151310.13173503071365109No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACATCGCCGCC149880.13049002976248777No Hit
TGGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTAC144700.12598016617715493No Hit
GGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCTC141840.1234901642748283No Hit
TGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTA141750.12341180757160823No Hit
GGTGGTGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG139870.12177502310434457No Hit
TGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTG138660.12072156076105255No Hit
TGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCCC138150.12027753944280548No Hit
TTTGGCTATAATTTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAG137990.12013823863708092No Hit
CAGGTTCCACACGAACGTGCGTTCAACGTGACGGGCGAGAGGGCGGCCCC137230.11947655980988922No Hit
TGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGG136050.11844921636767056No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA130540.11365204487053078No Hit
ACGAATGGTTTAGCGCCAGGTTCCACACGAACGTGCGTTCAACGTGACGG130010.11319061095156815No Hit
ACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACC126690.11030011923278339No Hit
GGGGTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCAT122190.10638228407177996No Hit
GGTCCGTTGATCAAAATATATTTGGGTCAATAAGATATGTTGATTTTACT121180.10550294773564364No Hit
TTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTTGCGGTACTA120300.10473679330415851No Hit
TGGGCCATTAGGTTTAATAGATTAAATAGATAAGTTATATAATTTAAGCT119930.10441466019092047No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119120.10370944986193985No Hit
CAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCC118390.1030738899358215No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118080.10280399462473015No Hit
GGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGTTGTTTTGTTAATTATT117660.10243833000970316No Hit
AGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGG116800.10168958817893363No Hit
GGCTTTCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAA116640.10155028737320905No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTG115660.1006970699381461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)