FastQCFastQC Report
Thu 6 Oct 2022
EGAF00007025586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007025586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11485935
Sequences flagged as poor quality0
Sequence length35-101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1546891.3467688960454678No Hit
GGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG914040.7957906779030179No Hit
TAACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT634090.5520577993868152No Hit
CGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG533110.4641415783738982No Hit
GAACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT467340.4068802409207435No Hit
TTTAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426040.37092322044308973No Hit
AAACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT363960.3168745078219579No Hit
GCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT355980.30992688013644515No Hit
TTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT341990.2977467659359034No Hit
GGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT317270.2762247914514578No Hit
GGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC294880.2567313849503763No Hit
GGTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG293300.2553557894938462No Hit
GGGGGATGGCGCTGGAGCGTCGGGCCCATACCCGGCCGTCGCCGGCAGTC280240.2439853612265784No Hit
TTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG276480.2407117922920511No Hit
GTGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG269370.23452161273766567No Hit
GACCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT263410.22933265772442554No Hit
TTTGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT256010.22288999545966437No Hit
GTTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG238030.20723606741636622No Hit
GCACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT232450.20237795181672194No Hit
CGGGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTTC228690.1991043828821946No Hit
GGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT223590.19466416969972405No Hit
ATTAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA214520.1867675552752127No Hit
TTACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT212850.18531360311546252No Hit
GGGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT209560.18244923029775112No Hit
CCCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT205190.17864457704139888No Hit
TTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC197320.17179271865982176No Hit
GTTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT176420.15359655091205024No Hit
TTAAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA171770.14954812124568007No Hit
GGCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT171690.14947847084281776No Hit
GCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG167520.14584794359362124No Hit
CGGCGGGGGGGCCGGCGGCGGCGGCGACTCTGGACGCGAGCCGGGCCCTT163560.14240024865193823No Hit
AGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG157120.1367933912215244No Hit
GGGGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC151260.13169149921186216No Hit
TATAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148960.12968905012957152No Hit
GGGAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148600.12937562331669125No Hit
CGCCCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT145150.12637194969325527No Hit
GTTAGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA144490.12579733386964143No Hit
TTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143050.12454362661812034No Hit
GTACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT142490.12405607379808435No Hit
GTGGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT138530.12060837885640133No Hit
GGACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT138480.12056484735461241No Hit
CAACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT136520.11885841248448646No Hit
GTGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT135550.11801390134978129No Hit
CCGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG133680.1163858231828754No Hit
TTTGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC133210.11597662706605949No Hit
CGGGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT133180.11595050816498613No Hit
GTTGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGTCTTGGGAAACGGGGT132180.11507987812920759No Hit
ATTGCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCT131820.11476645131632732No Hit
ATACCCCCGCCGGGCGTCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGT130890.11395676538305327No Hit
GGGGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCC120400.10482385630773637No Hit
TGGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG116740.10163735037678691No Hit
GAGGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGGGCGCGCGCCGCGG116040.10102790935174193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)